RNF43 p.G659fs 通过 PI3K/AKT/mTOR 信号通路和 HLA-E 上调导致 MSI-high 结直肠癌中 NK 细胞功能障碍
RNF43 p.G659fs leads to natural killer cell dysfunction in MSI-high colorectal cancer through PI3K/AKT/mTOR signaling and HLA-E up-regulation.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
microRNA-30c 靶向间充质干细胞中作为炎症细胞因子的白细胞介素 6 的作用：结直肠癌的一种治疗途径
英文原题：The role of microRNA-30c in targeting interleukin 6, as an inflammatory cytokine, in the mesenchymal stem cell: a therapeutic approach in colorectal cancer.
查看英文原题
The role of microRNA-30c in targeting interleukin 6, as an inflammatory cytokine, in the mesenchymal stem cell: a therapeutic approach in colorectal cancer.
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中文摘要
材料与方法
从小鼠骨髓分离并鉴定间充质基质细胞（MSC），再用携带miR-30C的慢病毒转导。收集转染后的MSC评估IL-6水平，并将CT-26细胞与MSC共培养，检测凋亡及上清液中的IL-6。
结果
与对照组相比，miR-30转导MSC的IL-6 mRNA表达和上清液蛋白水平下降。此外，凋亡分析显示，miR-30c转导MSC邻近的CT-26细胞发生凋亡。讨论与结论：研究显示，miR-30c下调与CRC进展及生存显著相关。本研究阐明miR-30c在CRC中的抗癌作用，并提出一个CRC治疗的新靶点。
展开英文摘要原文
MATERIALS AND METHODS
MSCs were isolated and identified from mice bone marrow and then transduced with lentiviruses containing miR-30C. Transfected MSCs were collected to evaluate IL-6 levels, CT-26 cells were also co-cultured with MSCs, and the effect of apoptosis and IL-6 on the supernatant was assessed.
RESULTS
Our result showed the expression of IL-6 mRNA and the level of protein were decreased in the supernatant of miR-30-transduced MSC cells compared to the control group. In addition, the rate of apoptosis was assessed, and the obtained data revealed the induction of apoptosis in CT-26 cells when they are in the vicinity of miR-30c-transduced MSCs. DISCUSSION AND CONCLUSION: We demonstrated that downregulation of miR-30c was significantly correlated with CRC progression and survival. So, the present study elucidated the anticancer effects of miR-30c in CRC and presented a novel target for CRC therapy.
论文信息
- 作者
- Mahjoor M、Afkhami H、Najafi M、Nasr A、Khorrami S
- 第一作者单位
- Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.Iran
- 通讯作者单位
- Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. khorami.s@iums.ac.ir.Iran
- 期刊
- Journal of cancer research and clinical oncology2023 Jul
- 原文标识
- PubMed 35876950 · DOI 10.1007/s00432-022-04123-w
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