CD4+ T 细胞转录因子在肿瘤微环境中的预后意义：来自乳腺癌 TIL（肿瘤浸润淋巴细胞）的见解

The tumor microenvironment (TME) influences breast cancer progression through immune system interactions, particularly by affecting the differentiation of CD4+ T cells.

This study aims to explore how the TME affects the expression of transcription factors-Foxp3, ROR t, GATA3, and T-bet-in tumor-infiltrating lymphocytes (TILs) and their implications for clinical outcomes. Forty-eight breast cancer patients were enrolled. Tumor tissues were collected, and quantitative real-time PCR was performed to assess the expression of transcription factors. Patients with positive lymphovascular invasion (LVI) and high tumor grades exhibited significantly elevated Foxp3 expression.

In contrast, ROR t expression decreased in patients with advanced disease. GATA3 levels correlated positively with LVI, tumor grade and advanced tumor stages, while T-bet expression was lower in patients with positive LVI, positive lymph node and larger tumor sizes.

Multivariate analysis identified ROR t as a risk factor for lymph node involvement and stage of disease, while GATA3 emerged as a protective factor. Receiver operating characteristic (ROC) analysis demonstrated GATA3's potential for late-stage detection, and ROR t as a reliable staging marker.

Our findings highlight the critical role of TIL-derived transcription factors in breast cancer progression and their potential as prognostic biomarkers, emphasizing the importance of TME-mediated T cell differentiation in shaping disease outcomes.