RNF43 p.G659fs 通过 PI3K/AKT/mTOR 信号通路和 HLA-E 上调导致 MSI-high 结直肠癌中 NK 细胞功能障碍
RNF43 p.G659fs leads to natural killer cell dysfunction in MSI-high colorectal cancer through PI3K/AKT/mTOR signaling and HLA-E up-regulation.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
从患者实体瘤生物制造免疫活性肿瘤芯片模型用于评估抗癌疗法的方案
英文原题：Protocol for the biofabrication of immunocompetent tumor-on-chip models from patient solid tumors for assessment of anticancer therapies.
查看英文原题
Protocol for the biofabrication of immunocompetent tumor-on-chip models from patient solid tumors for assessment of anticancer therapies.
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中文摘要
我们提出了一种从人类实体瘤生成具有免疫活性的3D肿瘤芯片模型的方案，能够比传统2D检测更准确地评估治疗反应。我们概述了自体肿瘤细胞、CD8+TIL（肿瘤浸润淋巴细胞）和癌症相关成纤维细胞的分离与培养，随后将其封装在微流控装置内的3D仿生基质中，并进行视频显微镜观察。该方案适用于其他肿瘤类型，包括乳腺癌和结肠癌。关于本方案使用和执行的完整细节，请参阅Veith等人1。
展开英文摘要原文
We present a protocol to generate immunocompetent 3D tumor-on-chip models from human solid tumors, enabling more accurate therapy response assessment than traditional 2D assays.
We outline the isolation and culture of autologous tumor cells, CD8 + tumor-infiltrating lymphocytes, and cancer-associated fibroblasts, followed by their encapsulation in a 3D biomimetic matrix within microfluidic devices and subsequent video microscopy. The protocol is adaptable to other tumor types, including breast and colon cancer. For complete details on the use and execution of this protocol, please refer to Veith et al. 1 .
论文信息
- 作者
- Nurmik M、Ladaigue S、Hofer I、Debache A、Veith I、Mechta-Grigoriou F、Descroix S、Zalcman G
- 第一作者单位
- Institut Curie, INSERM U1339, Stress and Cancer Laboratory, PSL Research University, Paris, France. Electronic address: martin.nurmik@curie.fr.France
- 通讯作者单位
- Institut Curie, INSERM U1339, Stress and Cancer Laboratory, PSL Research University, Paris, France. Electronic address: maria-carla.parrini@curie.fr.France
- 期刊
- STAR protocols2025 Sep 19
- 原文标识
- PubMed 40517390 · DOI 10.1016/j.xpro.2025.103895
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