结直肠癌肝转移灶含有不同的组织驻留记忆 CD8 T 细胞亚群，与术后不同的复发风险相关

Tissue-resident memory (T RM ) T cells have emerged as key players in cancer immunosurveillance, and their presence has been linked to a favorable clinical outcome in solid cancer patients. Liver metastases exhibit a highly immunosuppressive tumor microenvironment, however, the role and clinical impact of T RM cell infiltration in colorectal cancer remain elusive.

The expression of several tissue residency and activation biomarkers has been investigated on tumor-infiltrating lymphocytes isolated from 26 patients' colorectal cancer liver metastases (CRC liver metastases) and compared to 16 peripheral blood samples of patients with CRC liver metastases. Cytokine production was also evaluated in in vitro-activated T RM and non-T RM cells. The prognostic value of T RM cells was also assessed in a well-defined cohort of CRC liver metastases.

Here we identified two subsets of T RM cells expressing CD103 and/or CD69 showing significantly higher expression of tissue residency and activation biomarkers. CD103 + CD69 + T RM cells subset showed almost exclusive expression of tumor reactivity biomarkers PD-1 and CD39. Supporting this observation, CD103 + CD69 + T RM cells showed a more oligoclonal TCR repertoire. Both T RM subsets presented higher cytotoxic and functional capacity compared to non-T RM cells.

Our study shows that only the presence of CD103 + CD69 + T RM cells is associated with longer recurrence-free survival of colorectal cancer patients with liver metastases. Taken together, our work demonstrates the existence of a phenotypic heterogeneity of T RM cells in colorectal cancer liver metastases.

In this study, we identified a population of CD103 + CD69 + T RM cells exhibiting the characteristics of tumor reactivity and correlated with better patients' prognosis, with potential implications in optimal therapeutic strategies determination. Liver metastases of colorectal carcinoma contain CD103 + CD69 + and CD103 − CD69 + T RM subsetsCD103 + CD69 + T RM subset is enriched in antigen-experienced lymphocytes expressing PD1 and CD39The presence of CD103 + CD69 + T RM cells is associated with longer recurrence free survival.