基于 DNA 超分子水凝胶的保护性 NK 细胞储库用于增强三阴性乳腺癌治疗
Protective NK Cell Reservoir Based on DNA Supramolecular Hydrogel for Enhanced Triple-Negative Breast Cancer Therapy.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
乳腺导管原位癌中 T 细胞相关蛋白的表达
英文原题：Expression of T cell-related proteins in breast ductal carcinoma in situ.
查看英文原题
Expression of T cell-related proteins in breast ductal carcinoma in situ.
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中文摘要
本研究旨在考察原位导管癌（DCIS）肿瘤微环境免疫细胞中的 T 细胞亚型标志物表达，并评估其意义。研究构建了包含 191 例乳腺 DCIS 病例的组织芯片，并对 T 细胞亚型标志物（STAT3、STAT4、STAT-6 和 FOXP3）进行免疫组织化学染色。根据 ER、PR、HER2 和 Ki-67 检测结果，将 DCIS 分为管腔型、HER2 型和三阴性乳腺癌（TNBC）型；另根据间质 TIL 水平，将其分为低 TIL（<10%）和高 TIL（≥10%）型。
结果显示，54.6% 为管腔型，39.5% 为 HER2 型，5.9% 为 TNBC 型。STAT3 在管腔型肿瘤细胞中的阳性率较高；在 TNBC 免疫细胞中，STAT3、STAT4、STAT6 和 FOXP3 的阳性率均升高（p < 0.05）。与低 TIL 组相比，高 TIL 组免疫细胞的阳性率也更高（p < 0.001）。免疫细胞 T 细胞亚型标志物中，STAT3 与 STAT4 的一致性最高（总体一致率 83.7%，κ = 0.658），STAT4 与 FOXP3 的一致性最低（总体一致率 71.7%，κ = 0.370）。在免疫细胞中，STAT3 和 STAT4 阳性与坏死相关（p < 0.001）；在伴坏死的 DCIS 中，所有免疫细胞相关蛋白均为阴性与不良预后相关（p = 0.013）。
总之，DCIS 免疫细胞可表达多种 T 细胞亚型标志物，其中 TNBC 和高 TIL 型 DCIS 的阳性表达更高。
展开英文摘要原文
This study aims to explore the expression of T cell subtype markers within the immune cells constituting the tumor microenvironment of ductal carcinoma in situ (DCIS) and to assess its implications. A tissue microarray comprising 191 cases of breast DCIS was created, and immunohistochemistry staining for T cell subtype markers (STAT3, STAT4, STAT-6, and FOXP3) was conducted. The DCIS cases were categorized into luminal, HER-2, and TNBC (Triple-negative breast cancer) types based on ER, PR, HER-2, and Ki-67 results.
Additionally, they were classified as low-TIL (tumor-infiltrating lymphocytes) (<10%) or high-TIL ( 10%) types according to stromal TIL. Results revealed that 54. 6% were luminal, 39. 5% HER-2, and 5. 9% TNBC. STAT3 exhibited a high positivity rate in luminal-type tumor cells, while STAT3, STAT4, STAT6, and FOXP3 showed elevated positivity rates in TNBC immune cells ( p <0. 05).
Furthermore, a higher positivity rate was observed in high-TIL immune cells compared with low-TIL ( p <0. 001). The strongest agreement between T cell subtype markers in immune cells was found between STAT3 and STAT4 (OA=83. 7%, =0. 658), whereas the lowest was between STAT4 and FOXP3 (OA=71. 7%, =0. 370). In immune cells, STAT3 and STAT4 positivity correlated with necrosis ( p <0. 001), and the absence of positivity in all immune cell-related proteins in DCIS with necrosis was associated with poor prognosis ( p =0. 013).
In conclusion, the immune cells in DCIS exhibit positivity for diverse T cell subtype markers, with TNBC and high-TIL DCIS displaying heightened positivity.
论文信息
- 作者
- Shin E、Kim HM、Koo JS
- 第一作者单位
- Department of Pathology, Yonsei University College of Medicine, Seoul, South Korea.South Korea
- 通讯作者单位
- Department of Pathology, Yonsei University College of Medicine, Seoul, South Korea. kjs1976@yuhs.ac.South Korea
- 期刊
- Histology and histopathology2025 Apr
- 原文标识
- PubMed 39356080 · DOI 10.14670/HH-18-805