TP53 缺失通过上调 NF-κB-IFN-β-MHC-Ia 信号促进骨肉瘤对 NK 细胞的抵抗
TP53 Loss Elevates NF-κB-IFN-β-MHC-Ia Signaling to Promote NK Cell Resistance in Osteosarcoma.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
脂肪来源间充质干细胞移植改善老龄小鼠卵巢功能与卵母细胞质量
英文原题：Adipose-derived Mesenchymal Stem Cell Transplantation Improves Ovarian Function and Oocyte Quality in Aged Mice.
查看英文原题
Adipose-derived Mesenchymal Stem Cell Transplantation Improves Ovarian Function and Oocyte Quality in Aged Mice.
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研究概要
ASC 治疗可能有效改善高龄女性的生育能力。
研究思路结论见上方概要
背景
排卵数量和卵子质量的年龄相关性下降是女性不孕的主要原因，干细胞已被报道可有效促进组织再生。然而，当前的治疗方法仍不充分。本研究探讨了脂肪来源间充质干细胞（ASCs）对老龄小鼠卵巢功能的影响。
方法
在通过流式细胞术对ASCs进行表征后，研究了ASCs对排卵数量、受精率和囊胚形成率的影响。此外，还检查了卵巢卵泡数量和血清抗苗勒管激素（AMH）水平。使用标记有Kusabira Orange的ASCs来检查细胞给药后的位置。使用下一代RNA测序分析了排卵卵母细胞的质量。
结果
ASCs表现出间充质干细胞的特征，并分布于多种器官，包括卵巢基质。移植导致卵巢中卵母细胞数量和排卵增加，以及AMH值升高。遗传分析显示卵母细胞质量改善，受精率和囊胚形成率提高。
展开英文摘要原文
MATERIALS AND METHODS
Following the characterization of ASCs using flow cytometry, the effects of ASCs on the number of ovulations, fertilization rate, and blastocyst-formation rate were investigated. In addition, the number of ovarian follicles and serum anti-M llerian hormone (AMH) levels were examined. ASCs marked with Kusabira Orange were used to examine the location after cell administration. The quality of ovulated oocytes was analyzed using next-generation RNA sequencing.
RESULTS
ASCs showed characteristics of mesenchymal stem cells and were distributed to various organs, including the ovarian stroma. The transplantation resulted in increased number of oocytes and ovulation in the ovaries and increased AMH values. Genetic analysis revealed improved oocyte quality and increased fertilization and blastocyst-formation rates.
CONCLUSION
ASC therapy may be effective in improving fertility in older women.
论文信息
- 作者
- Hirakawa T、Yotsumoto F、Imi S、Ito N、Ishiwata K、Nakabayashi K、Watanabe A、Shirasu N
- 第一作者单位
- Department of Obstetrics & Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan; thira@fukuoka-u.ac.jp.Japan
- 通讯作者单位
- Department of Obstetrics & Gynecology, School of Medicine, Iwate Medical University, Iwate, Japan smiya3500@gmail.com.Japan
- 期刊
- Anticancer research2024 Aug
- 原文标识
- PubMed 39060058 · DOI 10.21873/anticanres.17180