负载端粒酶依赖性溶瘤腺病毒的同基因间充质干细胞增强抗转移疗效

Oncolytic adenoviruses have emerged as a promising therapeutic approach for cancer therapy.

However, systemic delivery of the viruses to metastatic tumors remains a major challenge. Mesenchymal stem cells (MSCs) possess tumor tropism property and can be used as cellular vehicles for delivering oncolytic adenoviruses to tumor sites. Since telomerase activity is found in ~90% of human carcinomas, but undetected in normal adult cells, the human telomerase reverse transcriptase gene (TERT) promoter can be exploited for regulating the replication of oncolytic adenoviruses.

Here, we evaluated the antitumor effects of syngeneic murine MSCs loaded with the luciferase-expressing, telomerase-dependent oncolytic adenovirus Ad. GS2 (MSC-Ad. GS2) and Ad. GS2 alone on metastatic MBT-2 bladder tumors. MSCs supported a low degree of Ad.

GS2 replication, which could be augmented by coculture with MBT-2 cells or tumor-conditioned medium (TCM), suggesting that viral replication is increased when MSC-Ad. GS2 migrates to tumor sites. MBT-2 cells and TCM enhanced viral replication in Ad. GS2-infected MSCs. SDF-1 is a stem cell homing factor.

Our results suggest that the SDF-1/STAT3/TERT signaling axis in MSCs in response to the tumor microenvironment may contribute to the enhanced replication of Ad. GS2 carried by MSCs.

Notably, we demonstrate the potent therapeutic efficacy of systemically delivered MSC-Ad. GS2 in pleural disseminated tumor and experimental metastasis models using intrapleural and tail vein injection of MBT-2 cells, respectively. Treatment with MSC-Ad. GS2 significantly reduced tumor growth and prolonged the survival of mice bearing metastatic bladder tumors. Since telomerase is expressed in a broad spectrum of cancers, this therapeutic strategy may be broadly applicable.