基于 DNA 超分子水凝胶的保护性 NK 细胞储库用于增强三阴性乳腺癌治疗
Protective NK Cell Reservoir Based on DNA Supramolecular Hydrogel for Enhanced Triple-Negative Breast Cancer Therapy.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
评估三阴性乳腺癌中 TIL（肿瘤浸润淋巴细胞）与 PD-L1 表达的关系：确定病理报告中 TILs 的最佳临界值
英文原题：Assessing the relationship between tumor-infiltrating lymphocytes and PD-L1 expression in triple negative breast cancer: Identifying optimal TILs cut-off value for pathologic reporting.
查看英文原题
Assessing the relationship between tumor-infiltrating lymphocytes and PD-L1 expression in triple negative breast cancer: Identifying optimal TILs cut-off value for pathologic reporting.
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研究概要
本研究确定了可预测 PD-L1 阳性的 TILs 截断值，提示各机构需要根据所选 PD-L1 克隆和治疗方法来调整这些阈值。仅在肿瘤边缘评估 TILs 可能忽视肿瘤免疫浸润的复杂性。虽然 TLS 的存在与较高的 PD-L1 表达相关，尤其是与 SP142 克隆，但其对 PD-L1 的确切预测价值仍有待阐明。与 22C3 相比，SP142 克隆表现出更高的阳性率。
研究思路结论见上方概要
背景
三阴性乳腺癌（TNBC）在诊断上存在复杂性，尤其是在评估TIL（肿瘤浸润淋巴细胞）（TILs）和程序性死亡配体1（PD-L1）表达方面。本研究旨在确定预测PD-L1表达的最佳TILs百分比截断值，并探讨TILs、PD-L1与三级淋巴结构（TLSs）之间的关系。
方法
分析141例TNBC病例，我们评估了TILs、PD-L1表达（克隆22C3和SP142）以及TLS的存在。
结果
我们确定了与PD-L1表达相关的TILs截断值（<20%、20-60%、60%）。TILs<20%时，无论是22C3还是SP142克隆，都很少表达PD-L1。TILs为60%时，两种克隆均显示PD-L1表达。TILs在20-60%范围内时，与SP142克隆的PD-L1表达相关，但与22C3克隆无关。仅在肿瘤边缘评估TILs会导致不准确，这凸显了需要对整个病灶的TILs进行全面评估。TLS的存在与更高的TIL百分比和PD-L1表达相关，尤其是与SP142。22C3与SP142克隆之间的差异（阳性率分别为15% vs. 50%）凸显了PD-L1检测的变异性。
展开英文摘要原文
BACKGROUND
Triple Negative Breast Cancer (TNBC) presents diagnostic complexities, particularly in evaluating Tumor-Infiltrating Lymphocytes (TILs) and Programmed Death-Ligand 1 (PD-L1) expression. This study aimed to identify optimal TILs percentage cut-offs predictive of PD-L1 expression and to investigate the relationship between TILs, PD-L1, and tertiary lymphoid structures (TLSs). METHOD: Analyzing 141 TNBC cases, we assessed TILs, PD-L1 expression (clones 22C3 and SP142), and TLS presence.
RESULTS
We identified TILs cut-offs (<20 %, 20-60 %, 60 %) correlating with PD-L1 expression. TILs <20 % rarely express PD-L1 with either 22C3 or SP142 clones. TILs 60 % demonstrate PD-L1 expression across both clones. TILs within the 20-60 % range correlate with PD-L1 expression using the SP142 clone, but not 22C3. Evaluating TILs solely at the tumor edge led to inaccuracies, highlighting the need for overall assessment of TILs throughout the entire lesion. TLS presence correlated with higher TIL percentages and PD-L1 expression, particularly with SP142. Discrepancies between 22C3 and SP142 clones (15 % vs. 50 % positivity, respectively) underscored the variability in PD-L1 detection.
CONCLUSION
This study identifies TILs cut-offs predictive of PD-L1 positivity, suggesting the need for institutions to tailor these thresholds based on the selected PD-L1 clone and treatment. Evaluating TILs solely at the tumor edge may overlook the complexity of tumor immune infiltration. While TLS presence correlates with higher PD-L1 expression, particularly with the SP142 clone, its exact predictive value for PD-L1 remains to be clarified. The SP142 clone exhibits higher positivity rates compared to 22C3.
论文信息
- 作者
- Suwannaphoom K、Soontornsit S、Wiwatwarayos K、Seneetuntigul P、Julimasart P
- 单位
- Institute of Pathology, Bangkok, Thailand. Electronic address: Kritpricetag@gmail.com.Thailand
- 期刊
- Annals of diagnostic pathology2024 Jun
- 原文标识
- PubMed 38513466 · DOI 10.1016/j.anndiagpath.2024.152294