基于 DNA 超分子水凝胶的保护性 NK 细胞储库用于增强三阴性乳腺癌治疗
Protective NK Cell Reservoir Based on DNA Supramolecular Hydrogel for Enhanced Triple-Negative Breast Cancer Therapy.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
中性粒细胞-淋巴细胞比值反映 TIL（肿瘤浸润淋巴细胞）和肿瘤相关巨噬细胞，并独立预测接受新辅助化疗的乳腺癌患者的不良结局
英文原题：Neutrophil-lymphocyte ratio reflects tumour-infiltrating lymphocytes and tumour-associated macrophages and independently predicts poor outcome in breast cancers with neoadjuvant chemotherapy.
查看英文原题
Neutrophil-lymphocyte ratio reflects tumour-infiltrating lymphocytes and tumour-associated macrophages and independently predicts poor outcome in breast cancers with neoadjuvant chemotherapy.
分数与星级只用于站内排序 —— 不代表疗效、安全性或个人适用性。
研究概要
NLR 与肿瘤坏死、TAMs 和 TILs 之间的关联说明了循环和局部免疫微环境之间的相互作用。晚期 NLR 是预后的强有力指标，可能有助于预后判断和疾病监测。
研究思路结论见上方概要
目的
中性粒细胞-淋巴细胞比值（NLR）是癌症相关炎症的全身反映，也是乳腺癌的预后标志物。对于局部肿瘤微环境，TIL（肿瘤浸润淋巴细胞）（TILs）和肿瘤相关巨噬细胞（TAMs）也与乳腺癌生存高度相关。本研究旨在探讨循环与局部免疫微环境之间的关系，并进一步阐明NLR在接受新辅助化疗（NAC）的乳腺癌患者中的预后作用。
方法
获取了一组接受NAC并随后手术的乳腺癌患者队列。回顾了临床数据。对活检（化疗前）和切除（化疗后）标本的组织学切片及CD8免疫组化进行了评估，以检测TILs和TAMs。
结果
共纳入146例患者。以2.6（化疗前NLR中位数）为截断值时，手术前与手术后NLR之间存在显著正相关（P < 0.001）。化疗前NLR与活检坏死（P = 0.027）、切除标本坏死（P = 0.021）及切除标本TAMs（P = 0.049）呈正相关。术后1年NLR与高肿瘤分期（P = 0.050）和低组织学分级（P = 0.008）相关。通过组织学评估和CD8免疫染色，在几乎所有时间点，NLR高值病例的TIL计数均较低（P < 0.050）。在多变量分析中，术后NLR是总生存期[OS；风险比（HR）= 9.524，P < 0.001]、乳腺癌特异性生存期（BCSS）（HR = 10.059，P = 0.001）和无病生存期（DFS；HR = 2.824，P = 0.016）的独立预测因子。
展开英文摘要原文
METHODS
A cohort of breast cancer patients receiving NAC with subsequent surgery was retrieved. Clinical data were reviewed. Histological slides and CD8 immunohistochemistry from biopsy (pre-chemotherapy) and excision (postchemotherapy) specimens were assessed for TILs and TAMs.
RESULTS
A total of 146 patients were included. There was a significant positive correlation between pre- and postsurgery NLR at a cut-off of 2.6 (median pre-chemotherapy NLR) (P < 0.001). NLR pre-chemotherapy was associated positively with necrosis on biopsy (P = 0.027) and excision (P = 0.021) and TAMs on excision (P = 0.049). NLR 1 year postsurgery was associated with high tumour stage (P = 0.050) and low histological grade (P = 0.008). TIL count was lower in NLR-high cases at almost all time-points by histological assessment and CD8 immunostaining (P < 0.050). In multivariate analysis, postsurgery NLR is an independent predictor for overall survival [OS; hazard ratio (HR) = 9.524, P < 0.001], breast cancer-specific survival (BCSS) (HR = 10.059, P = 0.001) and disease-free survival (DFS; HR = 2.824, P = 0.016).
CONCLUSIONS
The association between NLR with tumour necrosis, TAMs and TILs illustrates an interaction between the circulating and local immune microenvironment. Late NLR is a strong indicator of outcome and may be useful for prognostication and disease monitoring.
论文信息
- 作者
- Li JJX、Ni SYB、Tsang JYS、Chan WY、Hung RKW、Lui JWH、Ng SWY、Shum LK
- 单位
- Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.United Kingdom
- 期刊
- Histopathology2024 Apr
- 原文标识
- PubMed 38192219 · DOI 10.1111/his.15125