基于 DNA 超分子水凝胶的保护性 NK 细胞储库用于增强三阴性乳腺癌治疗
Protective NK Cell Reservoir Based on DNA Supramolecular Hydrogel for Enhanced Triple-Negative Breast Cancer Therapy.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
三阴性乳腺癌空芯针穿刺活检与手术标本间程序性死亡配体 1 状态的比较
英文原题：Comparison of Programmed Cell Death Ligand 1 Status between Core Needle Biopsy and Surgical Specimens of Triple-Negative Breast Cancer.
查看英文原题
Comparison of Programmed Cell Death Ligand 1 Status between Core Needle Biopsy and Surgical Specimens of Triple-Negative Breast Cancer.
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研究概要
在 TNBC 中，含 5 个肿瘤芯的 CNB 足以代表 TIL 水平和 PD-L1（22C3）状态。
研究思路结论见上方概要
目的
帕博利珠单抗目前用于治疗晚期三阴性乳腺癌（TNBC）以及联合新辅助化疗（NAC）治疗高危早期TNBC。TIL（肿瘤浸润淋巴细胞）水平和程序性细胞死亡配体1（PD-L1）状态是NAC和免疫检查点抑制剂治疗反应的预测因子。我们旨在研究TNBC中空芯针活检（CNB）的PD-L1状态是否能代表整个肿瘤。
方法
共纳入49例在2018年1月至2021年3月期间诊断为TNBC且未接受NAC而直接接受 upfront 手术的患者。在配对的CNB和切除标本中评估PD-L1表达（SP142和22C3克隆）和TIL。分析CNB与切除标本之间PD-L1状态和TIL水平的一致性。
结果
PD-L1阳性在切除标本中更常被观察到。CNB中TIL水平的总体可靠性良好[组内相关系数（ICC）=0.847，p <0.001]。PD-L1状态的一致性分别为良好和一般（SP142，=0.503，p <0.001；22C3，=0.380，p =0.010）。随着CNB核心数量的增加，可靠性和一致性也有所改善，尤其是从5个肿瘤核心开始（TIL，ICC=0.911，p <0.001；PD-L1 [22C3]，=0.750，p =0.028）。关于PD-L1（SP142），在5个肿瘤核心时未观察到进一步改善（=0.600，p =0.058）。
展开英文摘要原文
PURPOSE
Pembrolizumab is currently used to treat advanced triple-negative breast cancer (TNBC) and high-risk early TNBC with neoadjuvant chemotherapy (NAC). The tumor-infiltrating lymphocyte (TIL) level and programmed cell death ligand 1 (PD-L1) status are predictors of response to NAC and immune checkpoint inhibitor treatment. We aimed to investigate whether the PD-L1 status in core needle biopsies (CNBs) could represent the whole tumor in TNBC.
MATERIALS AND METHODS
A total of 49 patients diagnosed with TNBC who received upfront surgery without NAC between January 2018 and March 2021 were included. The PD-L1 expression (SP142 and 22C3 clones) and TIL were evaluated in paired CNBs and resected specimens. The concordance PD-L1 status and TIL levels between CNBs and resected specimens were analyzed.
RESULTS
PD-L1 positivity was more frequently observed in resected specimens. The overall reliability of TIL level in the CNB was good [intraclass correlation coefficient (ICC)=0.847, p <0.001]. The agreements of PD-L1 status were good and fair, respectively (SP142, =0.503, p <0.001; 22C3, =0.380, p =0.010). As the core number of CNB increased, the reliability and agreement also improved, especially from five tumor cores (TIL, ICC=0.911, p <0.001; PD-L1 [22C3], =0.750, p =0.028). Regarding PD-L1 (SP142), no further improvement was observed with 5 tumor cores ( =0.600, p =0.058).
CONCLUSION
CNBs with 5 tumor cores were sufficient to represent the TIL level and PD-L1 (22C3) status in TNBC.
论文信息
- 作者
- Choi H、Ahn SG、Bae SJ、Kim JH、Eun NL、Lee Y、Nahm JH、Jeong J
- 第一作者单位
- Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.South Korea
- 通讯作者单位
- Institute of Breast Cancer Precision Medicine, Yonsei University College of Medicine, Seoul, Korea. yooncha@yuhs.ac.South Korea
- 期刊
- Yonsei medical journal2023 Aug
- 原文标识
- PubMed 37488704 · DOI 10.3349/ymj.2023.0090
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