RNF43 p.G659fs 通过 PI3K/AKT/mTOR 信号通路和 HLA-E 上调导致 MSI-high 结直肠癌中 NK 细胞功能障碍
RNF43 p.G659fs leads to natural killer cell dysfunction in MSI-high colorectal cancer through PI3K/AKT/mTOR signaling and HLA-E up-regulation.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
通过生物信息学分析和体外实验鉴定结直肠癌的预后生物标志物及其与免疫浸润的相关性
英文原题：Identification of the prognostic biomarkers and their correlations with immune infiltration in colorectal cancer through bioinformatics analysis and in vitro experiments.
查看英文原题
Identification of the prognostic biomarkers and their correlations with immune infiltration in colorectal cancer through bioinformatics analysis and in vitro experiments.
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中文摘要
结直肠癌（CRC）是第三大确诊恶性肿瘤，也是第二大癌症死亡原因。目的是识别有助于CRC预后和靶向治疗的新枢纽基因。从基因表达综合数据库（GEO）中筛选出GSE23878、GSE24514、GSE41657、GSE81582。通过GEO2R鉴定差异表达基因（DEGs），并在DAVID中进行GO条目和KEGG通路富集。使用STRING构建并分析PPI网络，筛选出枢纽基因。基于癌症基因组图谱（TCGA）和基因型-组织表达（GTEx），在GEPIA中评估枢纽基因与CRC预后的关系。使用miRnet和miRTarBase构建枢纽基因的转录因子和miRNA-mRNA相互作用网络。在TIMER中分析枢纽基因与TIL（肿瘤浸润淋巴细胞）的关系。
在HPA中鉴定枢纽基因的蛋白水平。在体外鉴定枢纽基因在CRC中的表达水平及其对CRC细胞生物学效应的影响。作为枢纽基因，BIRC5、CCNB1、KIF20A、NCAPG和TPX2的mRNA水平在CRC中高表达，并具有优异的预后价值。BIRC5、CCNB1、KIF20A、NCAPG和TPX2与转录因子、miRNA、TIL（肿瘤浸润淋巴细胞）密切相关，提示它们参与CRC的调控。BIRC5在CRC组织和细胞中高表达，并促进CRC细胞的增殖、迁移和侵袭。BIRC5、CCNB1、KIF20A、NCAPG和TPX2是枢纽基因，可作为CRC中有前景的预后生物标志物。BIRC5在CRC的发生和进展中发挥重要作用。
展开英文摘要原文
Colorectal cancer (CRC) is the third most diagnosed malignancy and the second leading cause of cancer death. The objective was to identify novel hub genes that were helpful for prognosis and targeted therapy in CRC. GSE23878, GSE24514, GSE41657, GSE81582 were filtered from the gene expression omnibus (GEO). Differentially expressed genes (DEGs) were identified through GEO2R, which were enriched in the GO term and KEGG pathway in DAVID. PPI network was constructed and analyzed using STRING and hub genes were screened out. The relationships between hub genes and prognoses in CRC were evaluated in GEPIA based on the cancer genome atlas (TCGA) and genotype-tissue expression (GTEx). The transcription factors and miRNA-mRNA interaction networks for hub genes were performed using miRnet and miRTarBase.
The relationship between hub genes and tumor-infiltrating lymphocytes were analyzed in TIMER. The protein levels of hub genes were identified in HPA. The expression levels of hub gene in CRC and its effect on the biological effect of CRC cells were identified in vitro . As hub genes, the mRNA levels of BIRC5, CCNB1, KIF20A, NCAPG, and TPX2 were highly expressed in CRC and had excellent prognostic value.
The BIRC5, CCNB1, KIF20A, NCAPG, and TPX2 were closely associated with transcription factors, miRNAs, tumor-infiltrating lymphocytes, suggesting their involvement in the regulation of CRC. BIRC5 highly expressed in CRC tissues and cells, and promoted the proliferation, migration, and invasion of CRC cells. BIRC5, CCNB1, KIF20A, NCAPG, and TPX2 are hub genes that serve as promising prognostic biomarkers in CRC. BIRC5 plays an important role in the development and progression of CRC.
论文信息
- 作者
- Guo M、Li X、Li J、Li B
- 第一作者单位
- Department of Oncology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, China.China
- 通讯作者单位
- Department of General Surgery, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, China.China
- 期刊
- Heliyon2023 Jun
- 原文标识
- PubMed 37389063 · DOI 10.1016/j.heliyon.2023.e17101
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