CAR-T（CAR-T）细胞疗法在非肿瘤性疾病中的应用
Chimeric antigen receptor T (CAR-T) cell therapy in non-oncological diseases.
CAR-T（CAR-T）细胞在血液系统恶性肿瘤中的应用推动了这种免疫治疗形式的显著进展。
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
可注射光固化 CAR-T 细胞制剂增强小鼠黑色素瘤抗肿瘤活性
英文原题：Injectable and photocurable CAR-T cell formulation enhances the anti-tumor activity to melanoma in mice.
查看英文原题
Injectable and photocurable CAR-T cell formulation enhances the anti-tumor activity to melanoma in mice.
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中文摘要
CAR-T 细胞疗法作为一种新型个性化免疫疗法，在B细胞恶性肿瘤治疗中已显示出显著的临床疗效。然而，实体瘤中的进展受到肿瘤免疫抑制微环境中多种因素的阻碍。在本研究中，采用可注射且可光固化的甲基丙烯酰化明胶（GelMA）水凝胶作为CAR-T 细胞的储库，从而形成可注射CAR-T 甲基丙烯酰化明胶水凝胶递送（i-GMD）系统。根据我们的结果，该系统中的CAR-T 细胞能够正常扩增、持续释放，并在体外发挥抗肿瘤作用。与局部或静脉注射CAR-T 溶液相比，在肿瘤周围注射i-GMD基质表现出增强的抗肿瘤效果，并显著延长了小鼠的生存期。我们的研究结果表明，该治疗策略有望为不可切除肿瘤患者提供一种治疗方法。
展开英文摘要原文
The chimeric antigen receptor-T cells (CAR-T) therapy, as a novel personalized immunotherapy, has shown prominent clinical efficacy in the treatment of B-cell malignancies.
However, the progress in solid tumors was hindered by multiple elements in the tumor immunosuppressive microenvironment. In this study, an injectable and photocurable Gelatin Methacryloyl (GelMA) hydrogel was applied to be a depot of CAR-T cells, thus forming an injectable CAR-T Gelatin Methacryloyl hydrogels Delivery (i-GMD) system.
According to our results, CAR-T cells in this system could be normally amplified, sustained released, and play an anti-tumor role in vitro. When compared with local or intravenously injection of CAR-T solution, injection of i-GMD matrix around tumor demonstrated enhanced anti-tumor effect and markedly extended survival of mice.
Our research outcomes indicated that this therapeutic strategy might hopefully provide a treatment for patients with unresectable tumors.
论文信息
- 作者
- Zhou W、Lei S、Liu M、Li D、Huang Y、Hu X、Yang J、Li J
- 第一作者单位
- Department of Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, PR China.China
- 通讯作者单位
- Department of Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, PR China. Electronic address: weiwang@scu.edu.cn.China
- 文献类型
- 非美国政府资助研究
- 期刊
- Biomaterials2022 Dec
- 原文标识
- PubMed 36323072 · DOI 10.1016/j.biomaterials.2022.121872