CAR-T（CAR-T）细胞疗法在非肿瘤性疾病中的应用
Chimeric antigen receptor T (CAR-T) cell therapy in non-oncological diseases.
CAR-T（CAR-T）细胞在血液系统恶性肿瘤中的应用推动了这种免疫治疗形式的显著进展。
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
抗肿瘤及抗病毒靶点细胞治疗产品的免疫表型分析
英文原题：Immunophenotyping of Cellular Therapy Products Against Oncogenic and Viral Targets.
查看英文原题
Immunophenotyping of Cellular Therapy Products Against Oncogenic and Viral Targets.
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中文摘要
流式细胞术分析能够以最少的细胞数量，为细胞治疗产品的活力、表型及治疗潜力提供有价值的信息。本章介绍临床产品的分析方法，这些方法在实验方案中纳入了额外考量，以帮助保持染色的稳定性和可信度。这些方法可包括：评估表达嵌合抗原受体的 T 细胞（CAR-T）的转导效率，表征多克隆细胞培养物及输注后研究样本中多种淋巴细胞群体的频率，以及测定对抗原特异性刺激的活性或耗竭反应。本章还包括关于染色材料的储存与管理、补偿分析的频率以及参考标准的建议。
展开英文摘要原文
Flow cytometric analysis can provide valuable information on the viability, phenotype, and therapeutic potential of cell therapy products using a minimal amount of cell numbers. In this chapter, we describe methods for the analysis of clinical products that incorporate extra considerations within the protocols to help maintain consistency and confidence of staining.
This can include methods for evaluating the transduction efficiency for T cells expressing chimeric antigen receptors (CAR-T), characterizing the frequency of diverse lymphocyte populations within polyclonal cell cultures and postinfusion research samples, and measuring the activity or exhaustion in response to antigen-specific stimulation. This chapter also includes recommendations for storage and management of staining materials, frequency of compensation analysis, and reference standards.
论文信息
- 作者
- Lazarski CA、Datar AA、Hanley PJ、Srivastava SK
- 第一作者单位
- Center for Cancer and Immunology Research, Children's National Hospital, Washington, DC, USA.United States
- 通讯作者单位
- Center for Cancer and Immunology Research, Children's National Hospital, Washington, DC, USA. bio.sandeep@gmail.com.United States
- 期刊
- Methods in molecular biology (Clifton, N.J.)2027
- 原文标识
- PubMed 42814329 · DOI 10.1007/978-1-0716-5607-5_13