CAR-T（CAR-T）细胞疗法在非肿瘤性疾病中的应用
Chimeric antigen receptor T (CAR-T) cell therapy in non-oncological diseases.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
人皮肤类器官中 CAR-T 细胞对黑色素瘤免疫监视的建模
英文原题：Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.
查看英文原题
Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.
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中文摘要
T细胞介导疗法是黑色素瘤治疗领域的重要突破。然而，黑色素瘤T细胞疗法的临床应用仍面临障碍，限制了其潜力，因此临床前研究仍聚焦于疗法开发和优化。临床前研究技术包括使用异种移植小鼠模型进行体内评估、离体患者肿瘤组织培养，以及体外肿瘤球和黑色素瘤/T细胞共培养。但这些模型缺乏黑色素瘤皮肤癌适当的组织环境。本文介绍一种方法，将原代T细胞和嵌合抗原受体（CAR）T细胞与黑色素瘤细胞共同纳入由原代表皮角质形成细胞构建的人皮肤类器官中。
展开英文摘要原文
T cell-mediated therapies are major breakthroughs in the treatment of melanoma.
However, clinical hurdles in the application of T cell-mediated therapies in melanoma persist and limit their potential. As such, preclinical research efforts remain focused on their development and optimization. Preclinical research techniques include in vivo evaluation with xenograft mouse models, ex vivo patient tumor explant culture, and in vitro tumor spheroids and melanoma/T cell co-cultures.
However, these models lack the appropriate tissue context of melanoma skin cancer.
Here, we describe a technique for the incorporation of primary T cells and chimeric antigen receptor (CAR)-T cells with melanoma cells in human skin organoids derived from primary epidermal keratinocytes.
论文信息
- 作者
- Russell K、Adil A、Shivde R、McGrath B、Abuzer H、Cosgrove C、Juarez M、LePoole IC
- 第一作者单位
- Department of Dermatology, Northwestern University, Chicago, IL, USA.United States
- 通讯作者单位
- Department of Dermatology, Northwestern University, Chicago, IL, USA. bethany_perezwhite@rush.edu.United States
- 期刊
- Methods in molecular biology (Clifton, N.J.)2026 Jun 5
- 原文标识
- PubMed 42243456 · DOI 10.1007/7651_2026_711