RNF43 p.G659fs 通过 PI3K/AKT/mTOR 信号通路和 HLA-E 上调导致 MSI-high 结直肠癌中 NK 细胞功能障碍
RNF43 p.G659fs leads to natural killer cell dysfunction in MSI-high colorectal cancer through PI3K/AKT/mTOR signaling and HLA-E up-regulation.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
通过多参数流式细胞术表征不同 NK 细胞亚群的抗肿瘤活性
英文原题：Characterization of anti-tumor activity in different NK cell subsets by multiparametric flow cytometry.
查看英文原题
Characterization of anti-tumor activity in different NK cell subsets by multiparametric flow cytometry.
分数与星级只用于站内排序 —— 不代表疗效、安全性或个人适用性。
中文摘要
对人类NK细胞群体日益深入的分析已经产生了大量（或多或少）明确的亚群，这些亚群具有相当分化的功能特性。考虑到外周血NK细胞所提供的潜在临床前景，设计能够直接从外周血单个核细胞（PBMCs）中对大量细胞亚群进行功能评估的策略至关重要。在本章中，我们描述了一种简单的方法，用于定量经典NK细胞亚群的细胞毒性脱颗粒和IFN-γ产生，特别聚焦于NKG2C+“适应性”NK细胞。
展开英文摘要原文
The ever more insightful dissection of the NK cell population in humans has produced a plethora of (more or less) defined subpopulations with quite differentiated functional properties. Considering the potential clinical perspectives offered by peripheral blood NK cells, it is crucial to design strategies to functionally assess a large number of cell subsets directly from the peripheral mononuclear cells (PBMCs).
In this chapter, we describe a simple method to quantify cytotoxic degranulation and IFN-γ production in classical NK cell subsets, focusing, particularly on the NKG2C+ "adaptive" NK cells.
论文信息
- 作者
- Perrone C、Canevali P、Colamassaro V、Giusto E、Tamburini S、Ubezio G、Ferlazzo G、Vitale M
- 第一作者单位
- UO Patologia e Immunologia Sperimentale, IRCCS Ospedale Policlinico San Martino, Genova, Italy.Italy
- 通讯作者单位
- UO Patologia e Immunologia Sperimentale, IRCCS Ospedale Policlinico San Martino, Genova, Italy. Electronic address: raffaella.meazza@aomliguria.it.Italy
- 期刊
- Methods in cell biology2026
- 原文标识
- PubMed 42177051 · DOI 10.1016/bs.mcb.2026.02.022