CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
英文原题：An Altered Ratio of CD4+ and CD8+ T Lymphocytes in Cervical Cancer Tissue and Peripheral Blood as a Predictor of Prognostic Outcome: A Clinicopathological Study.
An Altered Ratio of CD4+ and CD8+ T Lymphocytes in Cervical Cancer Tissue and Peripheral Blood as a Predictor of Prognostic Outcome: A Clinicopathological Study.
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我们的研究表明，在局部晚期宫颈癌中，作为 TILs 主要组成部分的 CD4+和 CD8+ T 淋巴细胞在肿瘤组织中的数量显著低于外周血。未观察到 T 淋巴细胞亚群与宫颈癌主要预后因素之间存在关联。在 HPV 相关宫颈癌中观察到的 TILs 增强代表了一种具有前景治疗应用的重要改变。
我们的研究目的是比较宫颈癌患者宫颈肿瘤组织与外周血中的CD4+和CD8+ T淋巴细胞，并探讨它们与已知预后因素的关联。该研究还旨在探究TIL（肿瘤浸润淋巴细胞）（TILs）与患者HPV状态之间的关联。
在这项前瞻性研究中，纳入了42例局部晚期宫颈鳞状细胞癌患者。采用基于流式细胞术的方法，从同时采集的肿瘤组织和外周血样本制备的单细胞悬液中获取CD4+和CD8+ T淋巴细胞的百分比。对从肿瘤组织中提取的DNA进行分析，以检测HPV16（人乳头瘤病毒16型）和HPV18感染。比较了血液和肿瘤组织中的T淋巴细胞亚群。检验了T淋巴细胞与已知预后因素及肿瘤HPV状态之间的关联。
外周血中CD4+和CD8+ T细胞均显著高于肿瘤组织（p < 0.001）。由于肿瘤组织中CD8+细胞相对CD4+细胞减少较少，肿瘤组织中CD4/CD8比值与外周血相比发生逆转。T淋巴细胞亚群与宫颈癌已知预后参数之间未发现显著关联。HPV16感染肿瘤中CD4+和CD8+ T淋巴细胞浸润显著更高（p < 0.05）。HPV18阳性肿瘤中观察到CD4/CD8比值显著改变（p < 0.05）。
The aim of our study is to compare CD4+ & CD8+ T lymphocytes in the cervical tumor tissue with those in peripheral blood in patients with carcinoma cervix and to access thier association with known prognostic factors. The study also aims to investigate the association between tumor infiltrating lymphocytes (TILs) and the HPV status of the patients.
In this prospective study, 42 patients with locally advanced squamous cell carcinoma of cervix were included. Percentages of CD4+ and CD8+ T lymphocytes were obtained using flow cytometry-based method from single-cell suspension prepared from simultaneously collected tumor tissue and peripheral blood samples. DNA extracted from tumor tissue was analysed to detect HPV16 (Human Papillomavirus 16) and HPV18 infection. T lymphocyte subsets in blood and tumor tissue were compared. The association of T lymphocytes with known prognostic factors and HPV status of the tumor was examined. RESULT: Both CD4+ and CD8+ T cells were significantly higher in peripheral blood compared to tumor tissue (p < 0.001). The CD4/CD8 ratio was reversed in tumor tissue compared to peripheral blood due to relatively lower reduction of CD8+ cells compared to CD4+ cells in tumor tissue. No significant association of T lymphocyte subpopulation was found with known prognostic parameters of cervical cancer. CD4+ and CD8+ T lymphocyte infiltration was significantly higher in tumor with HPV16 infection (p < 0.05). A significant alteration of CD4/CD8 ratio was observed for HPV18 positive tumors (p < 0.05).
Our study demonstrates that both CD4+ and CD8+ T lymphocyte, which are major component of TILs, are significantly lower in tumor tissue compared to peripheral blood in locally advanced cervical cancer. No association was observed between T lymphocyte subpopulations and major prognostic factors of cervical cancer. The enhanced TILs observed in HPV-associated cervical cancer represents a significant alteration with promising therapeutic applications.
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