两阶段惯性微流控富集活化 T 细胞以实现无磁珠的嵌合抗原受体制备方案

CAR-T cell therapy is leading the way in the field of cancer cell immunotherapies due to its high success rates.

However, the manufacturing of CAR-T cells remains complex and expensive. T-cell enrichment from patient apheresis starting material is a key step in the manufacture but cellular impurities interfere with the ex vivo transduction of T-cells and their proliferation. Current enrichment methods including magnetic bead selection suffer from various limitations.

We report here a bead-less T-cell enrichment process through a two-stage procedure based on inertial microfluidics. Using apheresis like starting material samples from healthy donors, the dual-stage process showed an efficient 87% (SD 6%) enrichment and 80% (SD 30%) recovery of T-cells.

Validation of the process with ovarian cancer samples resulted in a T-cell purity 70% (SD 10%) from a starting purity of 48% (SD 6%) at a 64% (SD 4%) T-cell recovery. The two-stage inertial microfluidic process was also shown to have no detectable effect on the proliferation of the cells.