贝林妥欧单抗在 MRD 阳性/化疗延迟的儿童 B-ALL 中实现 MRD 清除并在复发/难治病例中显示高缓解率：多中心队列研究

Blinatumomab, a bispecific T-cell engager targeting CD3+ and CD19+, promotes T cell-mediated cytotoxicity against B-cell precursor acute lymphoblastic leukemia (B-ALL). While its efficacy is established in relapsed/refractory (R/R) disease, its role as preemptive therapy for minimal residual disease (MRD)-positive patients or those experiencing chemotherapy delays remains undefined. Predictors of treatment failure also require further investigation.

In this multicenter retrospective study, 105 patients who received blinatumomab were enrolled. Of these, 30 had R/R ALL, 21 were in complete remission (CR) with MRD positivity (CR-MRD pos ), and 54 experienced chemotherapy delays. Eight patients received blinatumomab directly as reinduction therapy and 22 patients received burden-reduction chemotherapy prior to blinatumomab. In total, 11 children were in R/R status and 40 were in CR-MRD pos before treatment. Patients were subsequently bridged to stem cell transplantation, chimeric antigen receptor T-cell therapy (CAR-T), or protocol continuation. Treatment response was analyzed across CR-MRD pos , R/R, and CR with MRD negativity (CR-MRD neg ). Immune reconstitution profiles (T-cell subsets, cytokine dynamics), cytogenetic markers, and clinical outcomes were assessed to identify predictors of treatment resistance.

The CR rate was 81.8% in R/R and 82.5% in CR-MRD pos patients (P = 1.000). Of 74 courses with CR-MRD neg , 73 remained MRD-negative during treatment. Univariate analysis revealed poor cytogenetics (P = 0.0001), CD19+ B-cell loss (P = 0.046), and BCR-ABL1 positivity (P = 0.002) as predictors of poor response. Cox regression analysis identified high MRD (P = 0.014), BCR/ABL1 (P = 0.065), and poor cytogenetics (P = 0.025) as independent risk factors. Blinatumomab significantly increased CD3+ T cells [0.96 (0.03-3.79) to 1.13 (0.26-7.74) 10 9 /L, P = 0.016], along with CD4+ [0.35 (0.01-1.39) to 0.47 (0.07-2.94) 10 9 /L] and CD8+ T cells [0.41 (0.01-2.39) to 0.56 (0.07-6.07) 10 9 /L] (P = 0.005 and P = 0.006, respectively).The 1-year event-free survival for CR-MRD neg , CR-MRD pos , and R/R patients was 97.8% 2.2%, 86.7% 6.2%, and 73.3% 8.1%, respectively (P = 0.001), while overall survival was 97.8% 2.2%, 100%, and 93.3% 4.6% (P = 0.029).

Blinatumomab effectively clears MRD as preemptive therapy and serves as a bridging strategy during chemotherapy delays in pediatric B-ALL, while maintaining high response rates in R/R cases.