超表面等离子体共振平台用于高通量、无标记、实时、长期监测体外癌症治疗评估

Improving real-time and long-term assessment of cellular behaviors in vitro is crucial for basic scientific research and drug development.

This study establishes a high-throughput, real-time, label-free, long-term cell analysis and drug screening platform based on metasurface plasmon resonance (MetaSPR) spectroscopy. This platform was applied to monitor cell proliferation, drug-target cell interactions, cell adhesion, and natural killer cell-mediated cell lysis.

The accuracy of the platform was validated by a linear relationship between the MetaSPR results and cell counting kit 8 (CCK-8) assay/imaging signals, providing a solid foundation for further experiments. This platform consistently monitors cell proliferation, drug-target cell interactions, and cell adhesion/spreading, yielding results comparable to those obtained using traditional CCK-8 and flow cytometry methods.

Additionally, the cytolytic activity of natural killer cells was evaluated by co-culturing them with target cells at varying effector:target ratios and monitoring their interactions. The results demonstrate that the spectroscopic MetaSPR detection system can quantitatively assess cytolytic activity mediated by immune cells.

The rapid response, cost-effectiveness, real-time monitoring capability, and non-invasive characteristics of this platform can enhance the understanding of complex cellular processes and create new possibilities for drug screening and clinical diagnostics.