PROTAC 工程化蛋白/DNA 纳米抗原是癌症免疫治疗中树突状细胞疫苗的有效增强剂
PROTAC-Engineered Protein/DNA Nanoantigen is a Potent Booster for Dendritic Cell Vaccines in Cancer Immunotherapy.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
乌拉尔甘草多糖金纳米颗粒作为抗原载体及 DC 疫苗的潜在佐剂
英文原题：Glycyrrhiza uralensis Polysaccharide Gold Nanoparticles as Antigen Carriers and Potential Adjuvant to DC Vaccines.
查看英文原题
Glycyrrhiza uralensis Polysaccharide Gold Nanoparticles as Antigen Carriers and Potential Adjuvant to DC Vaccines.
分数与星级只用于站内排序 —— 不代表疗效、安全性或个人适用性。
中文摘要
背景
宫颈癌是全球女性第四大死因，人乳头瘤病毒（HPV）被确定为主要致病因素。本研究探讨甘草多糖金纳米颗粒（GUPS-AuNPs）的免疫刺激活性和抗原递送效率，并评估以GUPS-AuNPs作为递送系统的HPV树突状细胞（DC）疫苗的抗肿瘤疗效。
方法
通过绿色还原法合成GUPS-AuNPs，并使用SEM、EDS、TEM、UV和FT-IR光谱等先进技术进行表征。以DCs作为主要实验模型，采用流式细胞术评估GUPS-AuNPs的免疫刺激活性和抗原递送效果。此外，建立TC-1荷瘤小鼠模型，评估GUPS-AuNPs辅助的HPV-DC疫苗的免疫刺激和抗肿瘤作用。
结果
合成的GUPS-AuNPs粒径为120.77 ± 3.13 nm，表面电荷为-11.9 ± 2.1 mV，稳定性优良。流式细胞术分析表明，GUPS-AuNPs显著增强DC成熟并促进T细胞增殖。此外，抗原递送实验显示，GUPS-AuNPs提高了DCs的抗原捕获能力。共聚焦成像证实，GUPS-AuNPs延长了抗原的细胞内滞留时间。体内研究表明，以GUPS-AuNPs为载体的HPV-DC疫苗有效抑制肿瘤生长，提高脾脏中CD4 + T和CD8 + T细胞数量，并诱导强烈的抗原特异性免疫应答。
结论
GUPS-AuNPs有效增强DC成熟和抗原递送，显著增强HPV疫苗触发的适应性免疫应答，从而抑制肿瘤进展。本研究将GUPS-AuNPs作为一种新型、安全、高效的抗原递送平台，在疫苗开发方面具有广阔前景。
展开英文摘要原文
Background : Cervical cancer is the fourth leading cause of death among women worldwide, with human papillomavirus (HPV) identified as a major contributing factor.
This study investigates the immunostimulatory activity and antigen delivery efficiency of Glycyrrhiza uralensis polysaccharide gold nanoparticles (GUPS-AuNPs) and assesses the antitumor efficacy of an HPV dendritic cell (DC) vaccine using GUPS-AuNPs as a delivery system.
Methods : GUPS-AuNPs were synthesized via a green reduction method and characterized using advanced techniques, including SEM, EDS, TEM, UV, and FT-IR spectroscopy. DCs served as the primary experimental model, with flow cytometry employed to evaluate the immunostimulatory activity and antigen delivery effectiveness of GUPS-AuNPs.
Additionally, a TC-1 tumor-bearing mouse model was established to assess the immunostimulatory and antitumor effects of the HPV-DC vaccine facilitated by GUPS-AuNPs. Results : The synthesized GUPS-AuNPs exhibited a particle size of 120. 77 ± 3. 13 nm, a surface charge of -11. 9 ± 2. 1 mV, and excellent stability. Flow cytometry analysis demonstrated that GUPS-AuNPs significantly enhanced DC maturation and promoted T cell proliferation.
Furthermore, antigen delivery experiments revealed that GUPS-AuNPs improved the antigen capture capabilities of DCs. Confocal imaging confirmed that GUPS-AuNPs extended the intracellular retention time of antigens. In vivo studies showed that the HPV-DC vaccine formulated with GUPS-AuNPs as carriers effectively suppressed tumor growth, elevated the populations of CD4 + T and CD8 + T cells in the spleen, and induced a robust antigen-specific immune response.
Conclusions : GUPS-AuNPs effectively enhance DC maturation and antigen delivery, significantly boosting the adaptive immune response triggered by HPV vaccines and leading to the inhibition of tumor progression. This research introduces GUPS-AuNPs as a novel, safe, and efficient antigen delivery platform with promising potential for vaccine development.
论文信息
- 作者
- Zhao Y、Song M、Dilixiati Y、Cai S、Maisaidi R、Aili P、Li J、Han L
- 单位
- Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, China.China
- 期刊
- Pharmaceutics2025 Sep 17
- 原文标识
- PubMed 41012548 · DOI 10.3390/pharmaceutics17091213
在 PubMed 查看 → 出版商原文（DOI） 全文 PDF（PMC）· 可下载 治疗专题与资料阅读指南 资料来源与翻译说明 报告译文或资料问题 →