CAR-T（CAR-T）细胞疗法在非肿瘤性疾病中的应用
Chimeric antigen receptor T (CAR-T) cell therapy in non-oncological diseases.
CAR-T（CAR-T）细胞在血液系统恶性肿瘤中的应用推动了这种免疫治疗形式的显著进展。
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
CAR-T 治疗的体外预测：用于 CAR-T 杀伤效应评估的仿生模型开发
英文原题：Prediction of CAR-T Therapy In Vitro: Development of Biomimetic Models for CAR-T Killing Effect Assessment.
查看英文原题
Prediction of CAR-T Therapy In Vitro: Development of Biomimetic Models for CAR-T Killing Effect Assessment.
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中文摘要
CAR-T（CAR-T）细胞疗法治疗实体瘤面临重大挑战，尤其是肿瘤异质性和复杂的肿瘤微环境所致问题。这些挑战凸显了建立可靠临床前评估模型的必要性。常用于评估 CAR-T 疗效的传统体内模型，往往不能充分代表人体免疫系统环境。近年来，研究者开发了多种体外模型，以更好地模拟体内肿瘤环境并评估 CAR-T 细胞疗效。本综述总结了常用体外模型的构建方法，包括传统二维细胞培养、球体、类器官、3D 生物打印和肿瘤芯片模型。综述特别关注器官芯片模型中仿生肿瘤微环境的构建，以评估 CAR-T 治疗实体瘤的效力和安全性。
展开英文摘要原文
Chimeric antigen receptor T (CAR-T) cell therapy faces significant challenges in treating solid tumors, particularly due to tumor heterogeneity and the complex tumor microenvironment. These challenges highlight the need for reliable preclinical assessment models. Traditional in vivo models, commonly used for evaluating CAR-T efficacy, often fail to adequately represent the human immune system context. In recent years, various in vitro models have been developed to better simulate the in vivo tumor environment and assess CAR-T cell efficacy.
This review summarizes the methodologies commonly employed in the construction of in vitro models, including traditional 2D cell cultures, spheroids, organoids, 3D bioprinting, and tumor-on-a-chip models. Special emphasis is placed on creation of biomimetic tumor microenvironment in the organ-on-a-chip models to evaluate the potency and safety of CAR-T therapy in the context of solid tumors CAR-T treatment.
论文信息
- 作者
- Sun X、Zhang Z、Zhao W、Xu S、Zhang J、Zhuansun X、Ye J、Han Y
- 单位
- State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, 210096, China.China
- 文献类型
- 综述 · 非美国政府资助研究
- 期刊
- Small (Weinheim an der Bergstrasse, Germany)2025 Sep
- 原文标识
- PubMed 40801090 · DOI 10.1002/smll.202503384