CD81 通过阻断 CD274/PD-L1 的选择性自噬降解驱动放射抵抗性胶质母细胞瘤的免疫逃逸
CD81 drives immune evasion in radioresistant glioblastoma by blocking selective autophagic degradation of CD274/PD-L1.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
脂肪来源间充质干细胞外泌体与培养上清的蛋白质组学及代谢组学分析
英文原题：Proteomic and Metabolomic Analyses of Adipose-derived Mesenchymal Stem Cell Exosomes and Culture Supernatants.
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Proteomic and Metabolomic Analyses of Adipose-derived Mesenchymal Stem Cell Exosomes and Culture Supernatants.
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研究概要
ASC 来源的培养上清液可通过外泌体蛋白和可溶性代谢物对受精卵发挥促分化作用，因此可能成为不孕症治疗的一种新选择。
研究思路结论见上方概要
背景
体外受精过程中受精卵的异常分化是不孕症的主要原因，脂肪间充质干细胞（ASCs）及其外泌体已被报道可促进受精卵分化；然而，其潜在机制仍不清楚。本研究旨在通过对ASC来源外泌体蛋白的蛋白质组学分析及培养上清液的代谢组学分析，阐明ASC来源外泌体如何促进受精卵分化。
方法
通过差速超速离心从ASC培养上清液中分离细胞外囊泡，提取蛋白质并使用相转移表面活性剂方案进行消化以用于LC-MS/MS分析，提取代谢物并在衍生化后使用GC-MS/MS进行分析。
结果
早期传代ASC来源的外泌体富含多种蛋白质，包括具有蛋白酶体活性的α和β蛋白酶体亚基。基因本体分析显示存在与跨膜转运和细胞质翻译相关的蛋白质。培养上清液的代谢组学分析显示，与甘氨酸、丝氨酸和甲硫氨酸代谢相关的氨基酸水平显著升高。
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MATERIALS AND METHODS
Extracellular vesicles were isolated from ASC culture supernatants via differential ultracentrifugation, proteins were extracted and digested using a phase-transfer surfactant protocol for LC-MS/MS analysis, and metabolites were analyzed using GC-MS/MS following extraction and derivatization.
RESULTS
Exosomes from early passage ASCs were enriched with various proteins, including alpha and beta proteasome subunits, which exhibit proteasome activity. Gene ontology analysis revealed the presence of proteins associated with transmembrane transport and cytoplasmic translation. Metabolomic profiling of culture supernatants revealed markedly elevated levels of amino acids associated with glycine, serine, and methionine metabolism.
CONCLUSION
ASC-derived culture supernatants can exert differentiation-promoting effects on fertilized eggs via exosomal proteins and soluble metabolites and may therefore be a novel option for infertility treatment.
论文信息
- 作者
- Hirakawa T、Kato T、Nakanishi Y、Urushiyama D、Miyata K、Baba T、Ohno H、Yasunaga S
- 第一作者单位
- Department of Obstetrics and Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan; thira@fukuoka-u.ac.jp.Japan
- 通讯作者单位
- Department of Obstetrics and Gynecology, School of Medicine, Iwate Medical University, Fukuoka, Japan; smiya3500@gmail.com.Japan
- 期刊
- Anticancer research2025 Aug
- 原文标识
- PubMed 40750428 · DOI 10.21873/anticanres.17703