经处理的破骨细胞与活破骨细胞相似，能够增强自然杀伤（sNK）细胞的功能

英文原题：Processed Osteoclasts Similar to Live Osteoclasts are Capable of Supercharging Natural Killer (sNK) Cells.

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Processed Osteoclasts Similar to Live Osteoclasts are Capable of Supercharging Natural Killer (sNK) Cells.

PubMed 2025/01/01（内容时间） Crit Rev Immunol Q4 · IF 1.8（JCR 2025）

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技术：NK 细胞地区：美国

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中文摘要

在本文中，我们比较了经处理的破骨细胞（pOCs）与活破骨细胞（OCs）扩增/超级激活NK细胞（sNK）的能力。与活OCs相比，pOCs诱导NK细胞扩增的水平相似。与活OC生成的sNK细胞相比，pOC生成的sNK细胞对口腔鳞状癌干细胞样细胞（OSCSCs）肿瘤的裂解能力较低。与活OC生成的sNK细胞相比，pOC生成的sNK细胞中IFN-γ分泌水平也略低。即使将活OCs培养上清加入pOC-sNK培养物中，与OC-sNK培养物相比，pOC-sNK培养物的细胞毒功能和IFN-γ分泌水平仍保持较低。与活OCs相比，pOCs在sNK细胞培养物中选择CD8+ T细胞的能力相当。总体而言，尽管活OCs的激活能力略优于经处理的破骨细胞，但pOCs因NK扩增周期更短、成本效益更高以及患者输注可及性更快，更适合用于sNK细胞的扩增。

展开英文摘要原文

In this paper, we compared the ability to expand/supercharge NK cells (sNK) by either processed osteoclasts (pOCs) or live osteoclasts (OCs). pOCs induced similar levels of cell expansion in NK cells compared to live OCs. pOC-generated sNK cells exhibited lower lysis of oral squamous carcinoma stem-like cells (OSCSCs) tumors in comparison to live OC-generated sNK cells.

Slightly lower levels of IFN-γ secretion were also observed in pOC-generated sNK cells when compared to live OC-generated sNK cells. Cytotoxic function and secretion levels of IFN-γ remained low in pOC-sNK cultures compared to OC-sNK cultures even after adding the supernatants harvested from live OCs to pOC-sNK cultures. pOCs were equally capable of selecting CD8+ T cells in sNK cell cultures when compared to live OCs.

Overall, even though live OCs are capable of activating slightly better than the processed osteoclasts, the use of pOCs is preferable for the expansion of sNK cells due to shorter NK expansion period, higher cost-effectiveness, and faster availability for patient infusion.

论文信息

作者: Sher E、Ko MW、Kaur K、Jewett A
第一作者单位: Division of Oral Biology and Medicine, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, University of California School of Dentistry, 10833 Le Conte Ave, 90095 Los Angeles, USA.United States
通讯作者单位: Division of Oral Biology and Medicine, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, University of California School of Dentistry, 10833 Le Conte Ave, 90095 Los Angeles, CA, USA; The Jonsson Comprehensive Cancer Center, UCLA School of Dentistry and Medicine, Los Angeles, CA, USA.United States
期刊: Critical reviews in immunology2025

原文标识: PubMed 40743764　·　DOI 10.1615/CritRevImmunol.2025056318

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