灵芝孢子油通过抑制类花生酸代谢通路调节荷瘤 H22 小鼠的免疫并限制肿瘤生长

The GLSO components were identified via UHPLC-Q-Orbitrap HRMS. A H22 cell subcutaneously transplanted tumor mouse model was constructed, and GLSO was preadministered. The antitumor efficacy of GLSO in hepatoma H22-bearing mice was evaluated according to tumor size, tumor growth curves, tumor inhibition rates and Ki67 level. T and B lymphocyte proliferation, delayed hypersensitivity, NK cell killing activity, macrophage phagocytotic activity and macrophage polarization, cytokine levels and CD69 molecule expression were detected to estimate immune function. Network pharmacology analysis, flow cytometry and Pro-DIA quantitative proteomics analysis were performed to investigate the potential mechanism of GLSO in tumor inhibition, which was verified by WB and RT-PCR.

Thirty-eight compounds including triterpenoids, fatty acids and esters, were identified from GLSO. Mice treated with GLSO showed the smaller initial and final tumor volumes and lower Ki67 expression, GLSO treatment could prevented tumor occurrence and inhibited tumor growth. Treatment with GLSO promoted a strong immune response including macroregulation in immune organs, enhancement of macrophage phagocytosis, NK cell cytotoxicity,T cells and B cells proliferation activity, delayed-type hypersensitivity reaction, reducing the production of M2 macrophages and regulation of cytokine secretion in hepatoma H22-bearing mice. Network pharmacology analysis and flow cytometry results showed that treatment with GLSO might have beneficial effects on improving the tumor immune microenvironment. Proteomics analysis showed that GLSO inhibited eicosanoid metabolism pathway, WB and RT-PCR re-check these results.

These findings support that GLSO enhances immunity in hepatoma H22-bearing mice and we first report that GLSO restricts tumor growth by inhibiting eicosanoid metabolism pathway.