CD81 通过阻断 CD274/PD-L1 的选择性自噬降解驱动放射抵抗性胶质母细胞瘤的免疫逃逸
CD81 drives immune evasion in radioresistant glioblastoma by blocking selective autophagic degradation of CD274/PD-L1.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
间充质干细胞来源外泌体作为多柔比星纳米药物载体联合 PVT1 siRNA 抑制胃癌进展
英文原题：Mesenchymal stem cell derived exosomes as Nanodrug carrier of doxorubicin combined with PVT1 siRNA inhibits the progression of gastric cancer.
查看英文原题
Mesenchymal stem cell derived exosomes as Nanodrug carrier of doxorubicin combined with PVT1 siRNA inhibits the progression of gastric cancer.
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研究概要
载多柔比星外泌体联合 si-PVT1 抑制了 GC 的进展。
中文摘要
材料与方法
采用CCK-8检测和免疫组织化学评估细胞增殖，测定外泌体及Exo-Dox的形态与大小。使用荧光显微镜观察游离外泌体和Exo-Dox在细胞中的分布。采用划痕愈合和Transwell实验评估细胞迁移和侵袭能力，并在异种移植瘤模型中验证Exo-Dox的保护作用。
结果
成功从MSC中分离并鉴定出外泌体。Exo-Dox粒径大于游离外泌体。酸性环境促进多柔比星释放，外泌体则促进细胞摄取多柔比星。与单独使用多柔比星相比，Exo-Dox对胃癌的抗肿瘤作用更强，可抑制胃癌细胞生长、迁移和侵袭。此外，Exo-Dox与si-PVT1联合治疗可明显抑制胃癌细胞的增殖、迁移和侵袭能力。在异种移植模型中，Exo-Dox联合si-PVT1可抑制肿瘤生长和转移。
结论
负载多柔比星的外泌体联合si-PVT1可抑制胃癌进展。
展开英文摘要原文
RESULTS
Exosomes were successfully isolated from MSCs and identified. The size of Exo-Dox was greater than that of free Exos. The acidic environment promoted the release of doxorubicin, and exosomes promoted the cellular uptake of doxorubicin. Compared with doxorubicin alone, Exo-Dox exhibited better antitumor effects on gastric cancer, inhibiting the growth, migration and invasion of gastric cancer cells. Additionally, combined therapy of Exo-Dox with si-PVT1 clearly suppressed the proliferation, migration and invasive ability of gastric cancer cells. Exo-Dox combined with si-PVT1 inhibited tumor growth and metastasis in a xenograft model.
CONCLUSION
Doxorubicin-loaded exosomes combined with si-PVT1 suppressed the progression of GC.
论文信息
- 作者
- Ouyang D、Li H、Luo K、Huang M、Yan S、Zhou L
- 单位
- Department of General Surgery, The Third Affiliated Hospital, Hengyang Medical School, University of South China, 377 Hengshan Road, Nanyue District, Hengyang City, Hunan Province 421900, PR China. Electronic address: ouyang0242dl@163.com.China
- 期刊
- Arab journal of gastroenterology : the official publication of the Pan-Arab Association of Gastroenterology2025 May
- 原文标识
- PubMed 40328564 · DOI 10.1016/j.ajg.2025.01.012