使用异体 NK 细胞的免疫治疗下调线粒体相关基因并抑制恶性脑膜瘤的 OXPHOS 系统

Malignant meningiomas (MM) are highly aggressive tumors and difficult to treat. Immunotherapy might be a promising treatment for cancers, including MM. This study assessed the anti-tumor effects of highly-purified human genuine-induced natural killer cells (GiNKs) on MM.

We evaluated the anti-tumor activity of allogeneic GiNKs with immortal human MM cell lines, IOMM-Lee and HKBMM. Inhibition of cell growth was assessed by apoptosis assay, fluorescent microscopic cytotoxic assays, and impedance-based real-time cell growth assays in vitro. We also confirmed the anti-tumor effect on MM in orthotopic xenograft murine models, and RNA sequencing analysis was performed using ex vivo MM cells from mice autopsy.

Allogeneic GiNKs had a positive effect on MM cells. Apoptosis assay and impedance-based real-time cell growth assays demonstrated the dose-dependent inhibitory growth effects of GiNKs on MM cell lines by inducing apoptosis (p < 0.0001 for both cell lines vs control). The intracranial administration of GiNKs prolonged the overall survival of the orthotopic xenograft MM murine models (p = 0.005 for both cell lines vs control). RNA sequencing analysis showed GiNKs downregulated mitochondrial-related genes, MT-ND1 and MT-RNR2, and inhibited the oxidative phosphorylation (OXPHOS) system of tumors derived from MM cells (p < 0.0001 for both cell lines vs control).

GiNKs promoted apoptosis of MM cells by downregulating mitochondrial-related genes and inhibiting the OXPHOS system. GiNKs might be a therapeutic option, in combination with other drugs such as immune checkpoint inhibitors, for patients with MM.