RNF43 p.G659fs 通过 PI3K/AKT/mTOR 信号通路和 HLA-E 上调导致 MSI-high 结直肠癌中 NK 细胞功能障碍
RNF43 p.G659fs leads to natural killer cell dysfunction in MSI-high colorectal cancer through PI3K/AKT/mTOR signaling and HLA-E up-regulation.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
宫颈癌术后放疗后循环淋巴细胞亚群的预后价值
英文原题：Prognostic Value of Circulating Lymphocyte Subsets in Cervical Cancer Following Postoperative Radiotherapy.
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Prognostic Value of Circulating Lymphocyte Subsets in Cervical Cancer Following Postoperative Radiotherapy.
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中文摘要
背景与目的
循环淋巴细胞亚群对接受术后放疗的宫颈癌患者的预后价值尚不明确。本研究旨在探讨这些淋巴细胞亚群在该患者群体中的预后意义。
方法
在101例宫颈癌患者接受术后放疗前采集外周血样本。采用流式细胞术测定淋巴细胞亚群的比例和绝对计数，包括总T细胞、CD4+ T细胞、CD8+ T细胞、自然杀伤（NK）细胞和B细胞。采用Kaplan-Meier法和Cox回归分析估计总生存期（OS）并确定关键预后因素。绘制受试者工作特征（ROC）曲线以评估预测准确性。
结果
生存分析表明，与NK细胞水平较高的患者相比，NK细胞比例降低（P = 0.02）或NK细胞计数减少（P = 0.01）的患者总生存期（OS）显著较差。在单因素Cox分析中，NK细胞比例（P = 0.025；HR，0.33；95% CI，0.12-0.87）和NK细胞计数（P = 0.015；HR = 0.28）均显著影响OS。在多因素分析中，CD4+ T细胞比例（P = 0.02；HR，0.08；95% CI，0.01-0.72）和NK细胞计数（P = 0.08；HR，0.11；95% CI，0.01-1.37）为独立预后因素。NK细胞计数预测1年、2年和3年生存率的ROC曲线下面积（AUC）分别为0.66、0.76和0.68。与诊断为早期阶段（特别是 IB3 和 IIA）的患者相比，诊断为 IIIC1 的患者 NK 细胞的绝对计数和比例均显著降低。
结论
我们的研究发现，治疗前循环 NK 细胞计数和比例可作为接受术后放疗的宫颈癌患者有前景的预后生物标志物。
展开英文摘要原文
Background and objective: The prognostic value of circulating lymphocyte subsets in cervical cancer patients receiving postoperative radiotherapy remains unclear.
This study aimed to explore the prognostic significance of these lymphocyte subsets in this patient population. Methods: Peripheral blood samples were collected from 101 cervical cancer patients prior to receiving postoperative radiotherapy. Flow cytometry was utilized to determine the proportions and absolute counts of lymphocyte subsets, including total T cells, CD4+ T cells, CD8+ T cells, natural killer (NK) cells, and B cells. The Kaplan-Meier method and Cox regression analysis were employed to estimate the overall survival (OS) and identify the key prognostic factors. Receiver operating characteristic (ROC) curves were generated to assess the predictive accuracy. Results: The survival analysis indicated that patients with a decreased proportion of NK cells ( P = 0. 02) or reduced NK cell counts ( P = 0. 01) exhibited significantly poorer overall survival (OS) compared to those with higher levels of NK cells.
In univariate Cox analysis, both the proportion of NK cells ( P = 0. 025; HR, 0. 33; 95% CI, 0. 12-0. 87) and NK cell counts ( P = 0. 015; HR = 0. 28) significantly influenced OS. In multivariate analysis, the proportion of CD4+ T cells ( P = 0. 02; HR, 0. 08; 95% CI, 0. 01-0. 72) and NK cell counts ( P = 0. 08; HR, 0. 11; 95% CI, 0. 01-1. 37) emerged as independent prognostic factors. The areas under the ROC curves (AUCs) for NK cell counts in predicting 1-, 2-, and 3-year survival were 0.
66, 0. 76, and 0. 68, respectively. Patients diagnosed with stage IIIC1 exhibited a significant reduction in both the absolute counts and the proportion of NK cells compared to those diagnosed with earlier stages, specifically IB3 and IIA. Conclusions: Our study found that pre-treatment levels of circulating NK cell counts and proportions serve as promising prognostic biomarkers for cervical cancer patients undergoing postoperative radiotherapy.
论文信息
- 作者
- Wang S、Zhao M、Gao Z、Yang X、Lu Y、Fu J
- 单位
- Department of Radiation Oncology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.China
- 期刊
- International journal of medical sciences2025
- 原文标识
- PubMed 40027185 · DOI 10.7150/ijms.107392
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