CAR-T（CAR-T）细胞疗法在非肿瘤性疾病中的应用
Chimeric antigen receptor T (CAR-T) cell therapy in non-oncological diseases.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
用于评估 CAR-T 细胞治疗的生物发光 AML-PDX 小鼠模型构建方案
英文原题：Protocol for the development of a bioluminescent AML-PDX mouse model for the evaluation of CAR T cell therapy.
查看英文原题
Protocol for the development of a bioluminescent AML-PDX mouse model for the evaluation of CAR T cell therapy.
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中文摘要
急性髓系白血病患者来源异种移植（AML-PDX）模型能够保留患者样本的复杂性和异质性，较细胞系模型具有优势。本文介绍在小鼠中建立生物发光AML-PDX模型、用于评估嵌合抗原受体（CAR）T细胞疗法的实验方案。文章描述AML-PDX细胞转导、植入、扩增及富集的步骤，随后详述体外和体内验证AML-PDX模型、用于评估CAR-T 细胞免疫治疗的流程。该方案的完整使用和执行细节请参阅Caulier等人的研究。
展开英文摘要原文
Patient-derived xenograft (PDX) models of acute myeloid leukemia (AML-PDX) offer advantages over cell line models by capturing the complexity and heterogeneity of patient-derived samples.
Here, we present a protocol for developing a bioluminescent AML-PDX model in mice to evaluate chimeric antigen receptor (CAR) T cell therapy.
We describe steps for transducing, engrafting, expanding, and enriching AML-PDX cells.
We then detail procedures for in vitro and in vivo validation of the AML-PDX model for the evaluation of CAR T cell immunotherapy. For complete details on the use and execution of this protocol, please refer to Caulier et al. 1 .
论文信息
- 作者
- Safont MM、Leitch C、Popa M、Gjerstad ME、Caulier B、Inderberg EM、Wälchli S、Gelebart P
- 第一作者单位
- Precision Oncology Research Group, University of Bergen, Bergen, Norway; Department of Clinical Science, University of Bergen, Bergen, Norway; Kinn Therapeutics, Bergen, Norway. Electronic address: mireia.safont@uib.no.Norway
- 通讯作者单位
- Precision Oncology Research Group, University of Bergen, Bergen, Norway; Department of Clinical Science, University of Bergen, Bergen, Norway; Department of Hematology, Haukeland University Hospital, Bergen, Norway; Centre for Pharmacy, Department of Clinical Science, University of Bergen, Bergen, Norway; Centre for Cancer Biomarkers (CCBIO), University of Bergen, Bergen, Norway. Electronic address: emmet.mc.cormack@uib.no.Norway
- 文献类型
- 非美国政府资助研究
- 期刊
- STAR protocols2024 Dec 20
- 原文标识
- PubMed 39673705 · DOI 10.1016/j.xpro.2024.103522
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