CAR-T（CAR-T）细胞疗法在非肿瘤性疾病中的应用
Chimeric antigen receptor T (CAR-T) cell therapy in non-oncological diseases.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
CAR-T 细胞针对肿瘤细胞的免疫突触形成与激活的活细胞成像方案
英文原题：Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells.
查看英文原题
Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells.
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中文摘要
免疫突触（IS）的形成决定T细胞的抗肿瘤活性。本文介绍一种方案，通过活细胞成像测量免疫突触大小和钙通量，以表征嵌合抗原受体（CAR）T细胞与肿瘤细胞之间的免疫突触形成。方案包括CAR-T 细胞制备、样本准备、图像采集及数据分析步骤。有关该方案用途和实施的完整细节，请参阅Chockley等、Ibanez等和Zoine等的研究。
展开英文摘要原文
Immune synapse (IS) formation determines T cell antitumor activity.
Here, we present a protocol for characterizing the IS formation between chimeric antigen receptor (CAR) T cells and tumor cells by measuring the IS size and calcium flux by live-cell imaging.
We describe steps for CAR T cell manufacturing, sample preparation, image acquisition, and data analysis. For complete details on the use and execution of this protocol, please refer to Chockley et al. , 1 Ibanez et al. , 2 and Zoine et al. 3 .
论文信息
- 作者
- Ibanez J、Hebbar N、Thanekar U、Yi Z、Houke H、Ward M、Nevitt C、Tian L
- 第一作者单位
- Department of Bone Marrow Transplantation and Cellular Therapy, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA.United States
- 通讯作者单位
- Department of Bone Marrow Transplantation and Cellular Therapy, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA. Electronic address: giedre.krenciute@stjude.org.United States
- 文献类型
- 非美国政府资助研究 · 美国 NIH 资助研究
- 期刊
- STAR protocols2024 Dec 20
- 原文标识
- PubMed 39488837 · DOI 10.1016/j.xpro.2024.103422
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