借力推动前列腺癌 CAR-T 细胞治疗进展
Piggybacking toward Progress for CAR T-Cell Therapy in Prostate Cancer.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
树突状细胞与细胞因子诱导的杀伤细胞共培养对前列腺癌细胞的免疫效应
英文原题：Immune Effect of Co-Culture of Dendritic Cells and Cytokine-Induced Killer Cells on Prostate Cancer Cells.
查看英文原题
Immune Effect of Co-Culture of Dendritic Cells and Cytokine-Induced Killer Cells on Prostate Cancer Cells.
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中文摘要
探讨树突状细胞（DC）与细胞因子诱导的杀伤细胞（CIK）共培养对前列腺癌（PCa）细胞的免疫效果。从健康献血者外周血中提取外周血单个核细胞（PBMCs），诱导并共培养DC和CIK细胞，观察DC、CIK及DC-CIK细胞/组的增殖和表型变化。通过向腹腔注射PC3 PCa细胞构建模型大鼠，将成功的50例分为阴性对照组、化疗组、DC组、CIK组和DC-CIK治疗组（每组10只），观察肿瘤进展。DC-CIK组白细胞介素-12（IL-12）（(103.67 ± 2.77) pg/mL）和干扰素-γ（IFN-γ）（(730.09 ± 23.52) pg/mL）的分泌浓度高于DC组和CIK组；在培养12至20天内，DC-CIK组中CIK的增殖高于CIK组。
DC-CIK组CD3 + / CD56 + 和CD8 + 的阳性率（PR）高于CIK组，而CD45RA + 的阳性率低于CIK组。在靶效比为10:1/20:1/50:1时，DC-CIK组的杀伤率高于DC组和CIK组（P < 0.05）。治疗后，化疗组、DC组和CIK组大鼠体重显著降低（P < 0.05），而阴性对照组和DC-CIK组未见显著变化（P > 0.05）。治疗25天后，DC-CIK组肿瘤大小显著小于阴性对照组、化疗组、DC组和CIK组；DC-CIK组肿瘤组织坏死面积也显著大于阴性对照组、化疗组、DC组和CIK组（P < 0.05）。DC与CIK共培养能显著促进CIK细胞增殖，增加IL-12和IFN-γ分泌，增强免疫细胞活性及抗肿瘤能力，在抗PCa肿瘤免疫治疗中显示出潜力。
展开英文摘要原文
It was to explore the immune outcome of co-culture of dendritic cells (DC) and cytokine-induced killer cells (CIK) on prostate cancer (PCa) cells. Peripheral blood mononuclear cells (PBMCs) were extracted from healthy blood donors. DC and CIK cells were induced and co-cultured. The proliferation and phenotypic changes of DC, CIK, and DC-CIK cells/groups were observed. Model rats were constructed by injecting PC3 PCa cells into the abdominal cavity. The successful 50 cases were divided into a negative control group, a chemotherapy group, a DC group, a CIK group, and a DC-CIK treatment group (each consisting of 10 rats) to observe tumor progression. The secreted concentrations of interleukin-12 (IL-12) ((103. 67 ± 2. 77) pg/mL) and interferon-γ (IFN-γ) ((730. 09 ± 23. 52) pg/mL) were higher in DC-CIK group as against DC and CIK groups; the proliferation of CIK was higher in DC-CIK group as against CIK within 12 to 20 days of culture. The positive rate (PR) of CD3 + / CD56 + and CD8 + was higher and that of CD45RA + was lower in DC-CIK group as against CIK.
The killing rate of the DC-CIK group was higher than that of the DC and CIK groups at a target effect ratio of 10:1/20:1/50:1 (P < 0. 05). After the treatment, the body weight of rats in the chemotherapy group, DC group, and CIK group was significantly reduced (P < 0. 05), while no significant changes were observed in the negative control group and DC-CIK group (P > 0. 05).
After 25 days of treatment, the tumor size in the DC-CIK group was significantly smaller compared to the negative control group, chemotherapy group, DC group, and CIK group; the necrotic area of the tumor tissue in the DC-CIK group was also significantly larger than that in the negative control group, chemotherapy group, DC group, and CIK group (P < 0.
05). Co-culture of DC and CIK is excellent in enhancing the proliferation of CIK cells, increasing the secretion of IL-12 and IFN-γ, and enhancing the activity of immune cells and anti-tumor ability, showing its potential in anti-PCa tumor immunotherapy.
论文信息
- 作者
- Li Y、Chen S、Liu S
- 第一作者单位
- Department of Urology Surgery, Tongxiang First People's Hospital, Tongxiang, 314500, Zhejiang, China.China
- 通讯作者单位
- Department of Urology Surgery, Tongxiang First People's Hospital, Tongxiang, 314500, Zhejiang, China. liaoyong96972884@163.com.China
- 期刊
- Cell biochemistry and biophysics2025 Jun
- 原文标识
- PubMed 39448420 · DOI 10.1007/s12013-024-01569-2