外周血中活化 CD4⁺ T 细胞比例与细胞因子释放综合征持续时间相关，并预测 CAR-T 细胞治疗后的临床结局

Objective Chimeric antigen receptor (CAR) T cell therapy is an emerging and effective therapy for relapsed or refractory diffuse large B cell lymphoma (R/R DLBCL). The characteristic toxicities of CAR T cell therapy include cytokine release syndrome (CRS) and prolonged cytopenia.

We investigated the factors associated with these complications after CAR T cell therapy by analyzing lymphocyte subsets following CAR T cell infusion. Methods We retrospectively analyzed peripheral blood samples on days 7, 14, and 28 after tisagenlecleucel (tisa-cel) infusion by flow cytometry at our institution between June 2020 and September 2022.

Patients Thirty-five patients with R/R DLBCL who received tisa-cel therapy were included. Results A flow cytometry-based analysis of blood samples from these patients revealed that the proportion of CD4 + CD25 + CD127 + T cells (hereafter referred to as "activated CD4 + T cells" ) among the total CD4 + T cells on day 7 after tisa-cel infusion correlated with the duration of CRS (r=0. 79, p<0. 01).

In addition, a prognostic analysis of the overall survival (OS) using time-dependent receiver operating characteristic curves indicated a significantly more favorable OS and progression-free survival of patients with a proportion of activated CD4 + T cells among the total CD4 + T cells <0. 73 (p=0. 01, and p<0. 01, respectively). Conclusion These results suggest that the proportion of activated CD4 + T cells on day 7 after tisa-cel infusion correlates with the CRS duration and predicts clinical outcomes after CAR T cell therapy.

Further studies with a larger number of patients are required to validate these observations.