CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
英文原题：Targeted nanotherapy platform mediated tumor-infiltrating CD8(+) T cell immune function effects for collaborative anti-tumor photothermal immunotherapy for cervical cancer.
Targeted nanotherapy platform mediated tumor-infiltrating CD8(+) T cell immune function effects for collaborative anti-tumor photothermal immunotherapy for cervical cancer.
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光热免疫治疗是一种创新的癌症治疗方法。它将免疫调节剂和光热剂相结合，二者均靶向肿瘤部位。该疗法利用光热转换产生的热量来损伤肿瘤细胞，同时释放肿瘤相关抗原。这一过程增强了肿瘤微环境（TME）中TIL（肿瘤浸润淋巴细胞）（TILs）的抗肿瘤免疫应答。光热免疫治疗作为一种新的癌症治疗方法正日益受到重视。由于其靶向疗效、全身副作用小以及治疗耐药风险降低，它成为当前的研究焦点。
本研究采用薄膜分散法制备脂质体（LIPO）作为复合药物载体。将临床使用的吲哚菁绿（ICG）用作光热剂（PTA），并将叶酸（FA）用作纳米复合材料的靶向剂。
我们将免疫佐剂CpG ODN包封在FA@LIPO@ICG纳米系统内，形成用于光热免疫治疗的靶向纳米颗粒（NPs）（FA@LIPO@ICG@CpG），并评估了药物包封率。FA@LIPO@ICG@CpG NPs表现出优异的水溶性，平均粒径范围为100至200 nm。
此外，我们研究了FA@LIPO@ICG@CpG NPs的光热特性。在808 nm激光照射下，FA@LIPO@ICG@CpG NPs的光热转换效率达到39.05%。随后，在808 nm激光激发下，我们通过流式细胞术分析了U14荷瘤小鼠的淋巴细胞亚群及其功能变化。该治疗方法表现出显著的抗肿瘤疗效。
因此，FA@LIPO@ICG@CpG NPs作为一种新颖且有前景的癌症治疗策略具有巨大潜力。
Photothermal immunotherapy is an innovative approach to cancer treatment. It combines immunomodulators and photothermal agents, both targeted to the tumor site. This therapy harnesses the heat generated by photothermal conversion to damage tumor cells while simultaneously releasing tumor-associated antigens.
This process enhances the anti-tumor immune response of tumor-infiltrating lymphocytes (TILs) within the tumor microenvironment (TME). Photothermal immunotherapy is gaining prominence as a new method for cancer treatment. It is a current focal point in research due to its targeted efficacy, minimal systemic side effects, and reduced risk of treatment resistance.
This study employed a thin-film dispersion method to fabricate liposomes (LIPO) as composite drug carriers. Indocyanine green (ICG) for clinical use was utilized as a photothermal agent (PTA), and folate (FA) was employed as a targeting agent for the nano-composite material.
We encapsulated the immunoadjuvant CpG ODN within the FA@LIPO@ICG nano-system, resulting in the formation of targeted nanoparticles (NPs) for photothermal immunotherapy (FA@LIPO@ICG@CpG), and assessed the drug encapsulation rate. FA@LIPO@ICG@CpG NPs demonstrated excellent water solubility with an average size ranging from 100 to 200 nm.
Furthermore, we investigated the photothermal properties of FA@LIPO@ICG@CpG NPs. Under 808 nm laser irradiation, the photothermal conversion efficiency of FA@LIPO@ICG@CpG NPs reached 39. 05%. Subsequently, under 808 nm laser excitation, we conducted an analysis of lymphocyte subpopulations and their functional changes in U14 tumor-bearing mice by using flow cytometry. This treatment approach demonstrated remarkable anti-tumor efficacy. Consequently, FA@LIPO@ICG@CpG NPs hold substantial promise as a novel and promising strategy in cancer therapy.
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