巧妙设计了一种 HER2 特异性巨噬细胞仿生多功能纳米平台，用于增强 HER2 阳性乳腺癌的生物-光热协同治疗

Photothermal therapy (PTT) has garnered extensive attention as an efficient strategy for cancer therapy. Unfortunately, there are currently no suitable photothermal agents (PTAs) capable of effectively treating HER2-positive breast cancer (HER2 + BC) due to the challenges in addressing blood circulation and tumor accumulation.

Here, we propose a HER2-specific macrophage biomimetic nanoplatform IR820@ZIF-8@EM (AMBP) for enhanced bio-photothermal therapy of HER2 + BC. An anti-HER2 antibody was expressed in engineered macrophages using the transmembrane expression technique. As an efficient PTAs, IR820 dyes were assembled into ZIF-8 as to develop a "nano-thermal-bomb". Homology modeling methods support that the expressed anti-HER2 antibody can specifically recognize the HER2 receptor.

Moreover, antibody-dependent cell-mediated cytotoxicity can also be induced in HER2 + BC cells by AMBP. In vitro fluorescence confocal imaging showed that AMBP promoted the uptake of HER2 + cancer cells while in vivo anti-tumor experiments demonstrated that AMBP efficiently accumulates in the tumor regions.

Finally, under spatiotemporally controlled near-infrared (NIR) irradiation, three of the six tumors were eradicated in AMBP-treated mice, demonstrating a safe and effective strategy.

In conclusion, our research opens a new paradigm for antibody-specific macrophage, and it is expected that these characteristics will have substantial clinical translation potential for BC treatment.