一组高分泌性 miRNA 与口腔鳞状细胞癌的淋巴结转移及不良预后相关

MicroRNAs (miRNAs) in oral squamous cell carcinoma (OSCC)-derived small extracellular vesicles (sEVs) play a pivotal role in modulating intercellular communications between tumor cells and other cells in the microenvironment, thereby influencing tumor progression and the efficacy of therapeutic interventions.

However, a comprehensive inventory of these secretory miRNAs in sEVs and their biological and clinical implications remains elusive.

This study aims to profile the miRNA content of OSCC cell line sEVs and computationally elucidate their biological and clinical relevance.

We conducted miRNA sequencing to compare the miRNA profiles of OSCC cells and their corresponding sEVs.

Our motif enrichment analysis identified specific sorting motifs that are implicated in either cellular retention or preferential sEV secretion. Target cell analysis suggested that the sEV miRNAs potentially interact with various immune cell types, including natural killer cells and dendritic cells.

Additionally, we explored the clinical relevance of these miRNAs by correlating their expression levels with TNM stages and patient survival outcomes. Intriguingly, our findings revealed that a distinct sEV miRNA signature is associated with lymph node metastasis and poorer survival in patients in TCGA-HNSC dataset. Collectively, this research furthers our understanding of the miRNA sorting mechanisms in OSCC and underscores their clinical implications.