川芎多糖通过 NF-κB 和 MAPKs 信号通路增强 RAW 264.7 细胞活化及 NK-92 细胞对结肠癌的细胞毒性

In this study, Cnidium officinale-derived polysaccharides were isolated and investigated for their immune enhancing and anticancer activities. The isolated crude and its fractions, such as F1 and F2, contain carbohydrates (51. 3-63. 1%), sulfates (5. 4-5. 8%), proteins (1. 5-7. 1%), and uronic acids (2. 1-26. 9%).

The molecular weight (M w ) of the polysaccharides ranged from 59. 9 to 429. 0 × 10 3 g/mol. The immunostimulatory activity of the polysaccharides was tested on RAW 264. 7 cells, and the results showed that the F2 treatment notably enhanced pro-inflammatory activity in RAW 264. 7 cells by increasing NO production and the expression of various cytokines.

Furthermore, the influence of polysaccharide treatment on natural killer cells (NK-92) anticancer activities was investigated using a colon cancer cell line (HCT-116). Crude polysaccharide and its fractions showed no direct cytotoxicity to NK-92 and HCT-116 cells.

However, the treatment of F2 showed an enhancement of NK-92 cells cytotoxicity against HCT-116 cells by upregulating the mRNA expression of IFN-γ, TNF-α, NKGp44, and granzyme-B. The western blot results showed that the induced RAW 264. 7 cells activation and NK-92 cells cytotoxicity occur via NF-κB and MAPK signaling pathways.

Overall, C. officinale-derived polysaccharides show potential as immunotherapeutic agents capable of enhancing pro-inflammatory macrophage signaling and activating NK-92 cells; thus, they could be useful for biomedical applications.