RNF43 p.G659fs 通过 PI3K/AKT/mTOR 信号通路和 HLA-E 上调导致 MSI-high 结直肠癌中 NK 细胞功能障碍
RNF43 p.G659fs leads to natural killer cell dysfunction in MSI-high colorectal cancer through PI3K/AKT/mTOR signaling and HLA-E up-regulation.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
基于综合生物信息学分析鉴定 HOXB9 用于预测头颈部鳞状细胞癌预后
英文原题：Identification of HOXB9 based on comprehensive bioinformatics analysis for predicting prognosis of head and neck squamous cell carcinoma.
查看英文原题
Identification of HOXB9 based on comprehensive bioinformatics analysis for predicting prognosis of head and neck squamous cell carcinoma.
分数与星级只用于站内排序 —— 不代表疗效、安全性或个人适用性。
中文摘要
评估HOXB9表达与头颈部鳞状细胞癌（HNSCC）预后及免疫浸润之间的相关性。通过TIMER2.0分析泛癌HOXB9表达。使用基因表达谱交互分析（GEPIA）和癌症基因组图谱（TCGA）数据库比较HNSCC与正常组织中的HOXB9表达数据。使用阿拉巴马大学伯明翰分校（UALCAN）数据库，基于临床病理特征（包括癌症分期、肿瘤分级和淋巴结分期）分析HOXB9在HNSCC亚组中的相对表达。使用GEPIA、TCGA-Portal、Kaplan-Meier Plotter和UALCAN数据库进行生存分析。使用TCGA数据鉴定与HOXB9共表达的基因，并通过GO和KEGG分析进行功能注释。使用STRING数据库和Cytoscape 3.7.1构建蛋白质-蛋白质相互作用网络。基于TCGA数据进行单样本基因集富集分析，以评估HOXB9与免疫浸润之间的相关性。使用TIMER 2.0数据库探讨HOXB9表达与多种癌症免疫浸润之间的相关性。HOXB9 mRNA在多种癌症中升高，并且与未配对正常组织（GEPIA中P < .05；TCGA中P < .0001）以及配对正常组织（TCGA中P < .0001）相比，在HNSCC组织中上调。
此外，在GEPIA和UALCAN数据库中，HOXB9表达与肿瘤恶性程度呈正相关（P < .05），且在两个数据库中与患者预后呈负相关（P < .05）。HOXB9高表达与aDCs、NK CD56bright细胞、NK细胞和Th2细胞浸润增加相关（P < .05），而 HOXB9 低表达与 DCs、iDCs、肥大细胞、中性粒细胞和 Th17 细胞比例增加相关（P < .05）。HOXB9 可能通过破坏免疫景观在 HNSCC 中发挥癌基因作用，是一个有前景的预后生物标志物和治疗靶点。
展开英文摘要原文
To evaluate the correlation between HOXB9 expression, and the prognosis and immune infiltration in head and neck squamous cell carcinoma (HNSCC). Pan-cancer HOXB9 expression was analyzed through TIMER2. 0. The HOXB9 expression data of HNSCC and normal tissues were compared using the gene expression profiling interactive analysis (GEPIA) and the cancer genome atlas (TCGA) databases. The University of Alabama at Birmingham (UALCAN) database was used to analyze the relative expression of HOXB9 in HNSCC subgroups based on clinicopathological features, including cancer stage, tumor grade and lymph node stage. Survival analysis was performed using GEPIA, TCGA-Portal, Kaplan-Meier Plotter, and UALCAN databases.
The genes co-expressed with HOXB9 were identified using TCGA data, and functionally annotated by GO and KEGG analyses. Protein-protein interaction network was constructed using the STRING database and Cytoscape 3. 7. 1. Single-sample gene set enrichment analysis was performed to assess the correlation between HOXB9 and immune infiltration based on TCGA data.
TIMER 2. 0 database was used to explore the correlation between HOXB9 expression and immune infiltration multiple cancers. HOXB9 mRNA is elevated in multiple cancers, and was upregulated in HNSCC tissues compared to non-paired (P < . 05 in GEPIA; P < . 0001 in TCGA) as well as paired (P < . 0001 in TCGA) normal tissues.
In addition, HOXB9 expression was positively correlated with tumor malignancy in the GEPIA and UALCAN databases (P < . 05), and negatively with patient prognosis in both databases (P < . 05). High HOXB9 expression was associated with increased infiltration of aDCs, NK CD56bright cells, NK cells, and Th2 cells (P < .
05), while low HOXB9 expression was associated with an increase in the proportion of DCs, iDCs, mast cells, neutrophils, and Th17 cells (P < . 05). HOXB9 likely functions as an oncogene in HNSCC by disrupting the immune landscape, and is a promising prognostic biomarker and therapeutic target.
论文信息
- 作者
- Li J、Ran H、Zeng X、Yang D、Zeng X、Zhang P
- 单位
- Department of Otorhinolaryngology, Longgang Otorhinolaryngology Hospital & Shenzhen Key Laboratory of Otorhinolaryngology, Shenzhen Institute of Otorhinolaryngology, Shenzhen, Guangdong, China.China
- 期刊
- Medicine2023 Sep 1
- 原文标识
- PubMed 37657018 · DOI 10.1097/MD.0000000000035035
在 PubMed 查看 → 出版商原文（DOI） 全文 PDF（PMC）· 可下载 治疗专题与资料阅读指南 资料来源与翻译说明 报告译文或资料问题 →