RNF43 p.G659fs 通过 PI3K/AKT/mTOR 信号通路和 HLA-E 上调导致 MSI-high 结直肠癌中 NK 细胞功能障碍
RNF43 p.G659fs leads to natural killer cell dysfunction in MSI-high colorectal cancer through PI3K/AKT/mTOR signaling and HLA-E up-regulation.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
NK 细胞对新城疫病毒感染肝细胞癌细胞应答的转录组分析
英文原题：Transcriptome Analysis of Natural Killer Cells in Response to Newcastle Disease Virus Infected Hepatocellular Carcinoma Cells.
查看英文原题
Transcriptome Analysis of Natural Killer Cells in Response to Newcastle Disease Virus Infected Hepatocellular Carcinoma Cells.
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中文摘要
当肿瘤细胞被新城疫病毒（NDV）感染后，自然杀伤（NK）细胞对肿瘤细胞的裂解作用增强，这可能与NK细胞活化效应增强有关。为更好地了解NK细胞活化所涉及的细胞内分子机制，我们分析了由NDV感染的肝细胞癌（HCC）细胞刺激的NK细胞（NDV组）和对照（NC组，由HCC细胞刺激的NK细胞）的转录组图谱。总体而言，与对照组相比，我们在NDV组的NK细胞中鉴定出1568个差异表达基因（DEGs），包括1389个上调基因和179个下调基因。功能分析显示，DEGs富集于免疫系统、信号传递、细胞生长、细胞死亡和癌症通路。值得注意的是，NDV感染后NK细胞中IFN家族的9个基因特异性增加，并被鉴定为HCC患者的潜在预后标志物。采用qRT-PCR实验证实了IFNG和其他8个重要基因的差异表达。本研究结果将增进我们对NK细胞活化分子机制的理解。
展开英文摘要原文
When tumor cells are infected by the Newcastle disease virus (NDV), the lysis of tumor cells by natural killer (NK) cells is enhanced, which may be related to the enhanced NK cell activation effect. To better understand the intracellular molecular mechanisms involved in NK cell activation, the transcriptome profiles of NK cells stimulated by NDV-infected hepatocellular carcinoma (HCC) cells (NDV group) and control (NC group, NK cells stimulated by HCC cells) were analyzed.
In total, we identified 1568 differentially expressed genes (DEGs) in the NK cells of the NDV group compared to the control, including 1389 upregulated and 179 downregulated genes. Functional analysis showed that DEGs were enriched in the immune system, signal transmission, cell growth, cell death, and cancer pathways.
Notably, 9 genes from the IFN family were specifically increased in NK cells upon NDV infection and identified as potential prognosis markers for patients with HCC. A qRT-PCR experiment was used to confirm the differential expression of IFNG and the other 8 important genes. The results of this study will improve our understanding of the molecular mechanisms of NK cell activation.
论文信息
- 作者
- Huang J、Zheng T、Liang Y、Qin Y、Wu X、Fan X
- 第一作者单位
- State Key Laboratory of Targeting Oncology, National Center for International Research of Bio-Targeting Theranostics, Guangxi Key Laboratory of Bio-Targeting Theranostics, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning 530021, China.China
- 通讯作者单位
- Department of Microbiology, School of Preclinical Medicine, Guangxi Medical University, Nanning 530021, China.China
- 文献类型
- 非美国政府资助研究
- 期刊
- Genes2023 Apr 10
- 原文标识
- PubMed 37107646 · DOI 10.3390/genes14040888
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