CAR-T（CAR-T）细胞疗法在非肿瘤性疾病中的应用
Chimeric antigen receptor T (CAR-T) cell therapy in non-oncological diseases.
CAR-T（CAR-T）细胞在血液系统恶性肿瘤中的应用推动了这种免疫治疗形式的显著进展。
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
用于治疗多发性骨髓瘤的第二代 CD38-CAR-T 细胞
英文原题：A second-generation CD38-CAR-T cell for the treatment of multiple myeloma.
查看英文原题
A second-generation CD38-CAR-T cell for the treatment of multiple myeloma.
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研究概要
这些结果提示 CD38-CAR-T 细胞是治疗 MM 的一种有前景的治疗策略。
中文摘要
背景
多发性骨髓瘤（MM）是侵袭性浆细胞恶性肿瘤，每年造成全球大量死亡；CAR转导T细胞疗法是治疗血液肿瘤的有前景免疫疗法。
方法
研究者构建第二代靶向CD38 CAR并制备CAR-T，评估其对MM细胞系的作用。
结果
体外实验中，CD38 CAR-T 对MM细胞系和原代MM细胞的细胞毒作用均强于对照T细胞。即使效靶比为1:100，仍可杀死超过50%的MM1.s和RPMI8226细胞；对原代MM细胞也有增强杀伤。CAR-T 可依赖靶抗原活化并产生多种细胞因子。体内实验显示，CAR-T 在异种移植小鼠模型中具有显著抗肿瘤效果。
结论
结果支持CD38 CAR-T 作为治疗MM的有前景策略。
展开英文摘要原文
BACKGROUND
Multiple myeloma (MM) is an aggressive plasma cell malignancy, causing a number of deaths worldwide every year. Chimeric antigen receptor (CAR) transduced T-cell therapy has been a promising immunotherapy against hematological malignancies.
METHODS
In this study, we developed a second-generation CAR construct and generated CAR-T cells targeting CD38 molecule. Then effects of CAR-T cells against MM cell lines were evaluated.
RESULTS
CD38-CAR-T cells showed higher cytotoxicity to MM cell lines and primary MM cells than that of control T cells in vitro. Over 50% MM1.s and RPMI8226 cells were killed by CAR-T cells even at effector to target ratio of 1:100. CAR-T cells also showed an enhanced cytotoxicity against primary MM cells. CAR-T cells could be activated and produced a variety of cytokines in a target-dependent manner. In vivo test indicated that CAR-T cells also showed significant antitumor effect on xenograft mice models.
CONCLUSION
These results indicated a promising therapeutic strategy of CD38-CAR-T cells against MM.
论文信息
- 作者
- Li H、Li J、Wu J、Shi Z、Gao Y、Song W、Li J、Li Z
- 单位
- Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.China
- 文献类型
- 非美国政府资助研究
- 期刊
- Cancer medicine2023 May
- 原文标识
- PubMed 37039305 · DOI 10.1002/cam4.5818
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