RNF43 p.G659fs 通过 PI3K/AKT/mTOR 信号通路和 HLA-E 上调导致 MSI-high 结直肠癌中 NK 细胞功能障碍
RNF43 p.G659fs leads to natural killer cell dysfunction in MSI-high colorectal cancer through PI3K/AKT/mTOR signaling and HLA-E up-regulation.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
评估 KIR3DL3 阻断在基于 NK 细胞的异种肺肿瘤模型中的抗肿瘤活性的方案
英文原题：Protocol for evaluating antitumor activity of KIR3DL3 blockade in an NK cell-based xenogeneic lung tumor model.
查看英文原题
Protocol for evaluating antitumor activity of KIR3DL3 blockade in an NK cell-based xenogeneic lung tumor model.
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中文摘要
人杀伤细胞免疫球蛋白样受体，三个Ig结构域和长胞质尾（KIR3DL3）表达于自然杀伤（NK）细胞，是新发现的B7家族成员HERV-H LTR关联蛋白2（HHLA2）的抑制性受体。在此，我们总结了KIR3DL3+人NK细胞的分离和扩增，以及内部抗KIR3DL3单克隆抗体（mAb）的体外功能表征。我们还描述了一种基于人NK细胞的异种肺肿瘤模型，用于在体内测试KIR3DL3阻断的治疗活性。关于本方案使用和执行的完整细节，请参阅Wei et al.（2021）。
展开英文摘要原文
Human killer cell immunoglobulin-like receptor, three Ig domains and long cytoplasmic tail (KIR3DL3) is expressed on natural killer (NK) cells and is a newly identified inhibitory receptor for B7 family member HERV-H LTR-associating protein 2 (HHLA2).
Here, we summarize the isolation and expansion of KIR3DL3 + human NK cells, and in vitro functional characterization of in-house anti-KIR3DL3 monoclonal antibody (mAb).
We also describe a human NK cell-based xenogeneic lung tumor model for testing the therapeutic activity of KIR3DL3 blockade in vivo . For complete details on the use and execution of this protocol, please refer to Wei et al. (2021).
论文信息
- 作者
- Ren X、Corrigan DT、Zang X
- 第一作者单位
- Department of Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.United States
- 通讯作者单位
- Department of Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Oncology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Urology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Electronic address: xingxing.zang@einsteinmed.edu.United States
- 文献类型
- 美国 NIH 资助研究 · 美国政府（非公共卫生署）资助研究 · 非美国政府资助研究
- 期刊
- STAR protocols2022 Dec 16
- 原文标识
- PubMed 36386885 · DOI 10.1016/j.xpro.2022.101818
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