间皮素作为癌症免疫治疗的生物标志物和治疗靶点
Mesothelin as Biomarker and Therapeutic Target for Immunotherapy in Cancer.
癌症仍是一个关键的全球健康问题，原因在于发现晚、耐药和高死亡率。
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
建立一株源自 Borrmann 2 型原发肿瘤的高微卫星不稳定性胃癌细胞系 OCUM-13
英文原题：Establishment of a gastric cancer cell line with high microsatellite instability, OCUM-13, derived from Borrmann type-2 primary tumor.
查看英文原题
Establishment of a gastric cancer cell line with high microsatellite instability, OCUM-13, derived from Borrmann type-2 primary tumor.
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中文摘要
微卫星不稳定性（MSI）的胃癌（GC）已被报道对免疫治疗敏感，然而部分MSI的GC病例仍对免疫治疗耐药。显示MSI的癌细胞系可能有助于免疫治疗机制的分析，但迄今为止可用的MSI胃癌细胞系仅有少数。在本研究中，我们从一例伴有丰富TIL（肿瘤浸润淋巴细胞）的原发性胃癌中建立了一株独特的MSI胃癌细胞系OCUM-13。MSI检测表明，OCUM-13细胞以及原发肿瘤在5个微卫星位点中有超过3个显示条带移位，提示OCUM-13确实具有高度MSI。将OCUM-13细胞皮下接种至小鼠后形成了肿瘤。胰岛素样生长因子1受体抑制剂降低了OCUM-13细胞的生长。新建立的MSI细胞系OCUM-13可能有助于MSI胃癌癌症治疗的分析。
展开英文摘要原文
Gastric cancer (GC) with microsatellite instability (MSI) has been reported to be sensitive to immunotherapy, however some of GC cases with MSI remain resistant to immunotherapy. Cancer cell lines showing MSI might be useful for the analysis of mechanisms of immunotherapy, while only a few GC cell lines with MSI are available so far. In this study, we established a unique GC cell line with MSI, OCUM-13, from a primary GC with abundant tumor-infiltrating lymphocytes.
MSI assay indicated that OCUM-13 cells as well as the primary tumor showed a band shift in more than 3 of 5 microsatellite loci, suggesting that OCUM-13 did have high MSI. The subcutaneous inoculation of OCUM-13 cells into mice performed tumor formation. Insulin-like growth factor 1 receptor inhibitor decreased the growth of OCUM-13 cells. The newly established cell line with MSI, OCUM-13, might be useful for the analysis of cancer therapy for GC with MSI.
论文信息
- 作者
- Yamamoto Y、Masuda G、Kushiyama S、Maruo K、Tsujio G、Sera T、Sugimoto A、Nishimura S
- 单位
- Molecular Oncology and Therapeutics, Osaka Metropolitan University Graduate School of Medicine, Osaka, Japan.Japan
- 文献类型
- 非美国政府资助研究
- 期刊
- Cancer medicine2023 Mar
- 原文标识
- PubMed 36324252 · DOI 10.1002/cam4.5403
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