CD81 通过阻断 CD274/PD-L1 的选择性自噬降解驱动放射抵抗性胶质母细胞瘤的免疫逃逸
CD81 drives immune evasion in radioresistant glioblastoma by blocking selective autophagic degradation of CD274/PD-L1.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
SPTSSA 是胶质母细胞瘤的预后标志物，与肿瘤浸润免疫细胞和氧化应激相关
英文原题：SPTSSA Is a Prognostic Marker for Glioblastoma Associated with Tumor-Infiltrating Immune Cells and Oxidative Stress.
查看英文原题
SPTSSA Is a Prognostic Marker for Glioblastoma Associated with Tumor-Infiltrating Immune Cells and Oxidative Stress.
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研究概要
这些发现表明，SPTSSA 表达可能作为胶质瘤的预后生物标志物以及新型胶质瘤治疗的潜在靶点。
研究思路结论见上方概要
背景
SPTSSA 编码丝氨酸棕榈酰转移酶的小亚基 A。它催化鞘脂鞘氨醇长链碱基骨架的形成。其胶质瘤预后和肿瘤浸润免疫细胞中的作用仍不清楚。
方法
我们利用GEPIA和CGGA数据库分析了SPTSSA的表达及其与临床预后的关联。随后，通过GSEA识别SPTSSA的相关生物学功能。使用CIBERSORT和TIMER探究了SPTSSA表达与肿瘤免疫浸润之间的相关性。最后，采用IHC和IF验证了其预后价值及与免疫浸润的相关性。
结果
SPTSSA在弥漫性胶质瘤中的表达较正常组织显著上调，并与GEPIA和CGGA数据库中的不良生存相关。随后，我们鉴定了与SPTSSA表达相关的生物学过程和信号通路。结果显示，SPTSSA富集于氧化应激等GO条目中。最后，我们通过IHC显示SPTSSA表达与肿瘤浸润免疫细胞和总生存期显著相关。
展开英文摘要原文
BACKGROUND
SPTSSA encodes the small subunit A of serine palmitoyltransferase. It catalyzes the formation of sphingoid long-chain base backbone of sphingolipids. Its role in glioma prognosis and tumor-infiltrating immune cells remains unclear.
METHODS
We analyzed SPTSSA expression and association with clinical prognosis using GEPIA and CGGA database. Then, GSEA was performed to identify relevant biological functions of SPTSSA . The correlations between SPTSSA expression and tumor immune infiltrates were investigated using CIBERSORT and TIMER. Finally, IHC and IF were performed to confirm the value of prognosis and the correlation with immune infiltration.
RESULTS
SPTSSA expression was significantly upregulated in diffuse glioma compared to normal tissues and associated with poor survival in GEPIA and CGGA database. Then, we identified biological processes and signaling pathways associated with SPTSSA expression. The result showed that SPTSSA enriched in the GO term like oxidative stress. Finally, we showed that SPTSSA expression was significantly associated with tumor-infiltrating immune cells and overall survival via IHC.
CONCLUSION
These findings suggest that SPTSSA expression might be used as a prognostic biomarker for glioma and potential target for novel glioma therapy.
论文信息
- 作者
- Wang Z、Ge X、Shi J、Lu B、Zhang X、Huang J
- 单位
- Department of Clinical Biobank & Institute of Oncology, Affiliated Hospital of Nantong University, Nantong, China 226000.China
- 期刊
- Oxidative medicine and cellular longevity2022
- 原文标识
- PubMed 36062185 · DOI 10.1155/2022/6711085
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