CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
系统分析 ITPR1 在乳腺癌中的表达和预后价值及其与肿瘤浸润免疫细胞的相关性
英文原题：Systematic analysis of the expression and prognostic value of ITPR1 and correlation with tumor infiltrating immune cells in breast cancer.
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Systematic analysis of the expression and prognostic value of ITPR1 and correlation with tumor infiltrating immune cells in breast cancer.
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研究概要
ITPR1 在乳腺癌中低表达。ITPR1 高表达提示患者预后良好。ITPR1 与免疫浸润水平相关，尤其是在 BRCA-Basal 患者中。所有研究结果表明，ITPR1 可能影响乳腺癌预后并参与免疫调节。总之，ITPR1 可能是乳腺癌治疗的潜在靶点。
研究思路结论见上方概要
ITPR1是自噬的关键基因，但其生物学功能尚不清楚，关于ITPR1基因表达与乳腺癌发生发展相关性的研究较少。
通过Oncomine和TIMER等在线数据库分析ITPR1的表达。使用Kaplan-Meier plotter等数据库评估ITPR1对临床预后的影响。通过免疫组织化学检测145例乳腺癌和30例癌旁正常组织中ITPR1的表达。采用统计学分析评估异常表达蛋白的临床相关性和预后意义。并使用Western Blot检测乳腺癌组织和细胞之间ITPR1的表达。TIMER数据库研究了ITPR1与癌症免疫浸润之间的关系。并使用ROC plotter数据库预测ITPR1对乳腺癌患者化疗、内分泌治疗和抗HER2治疗的反应。
与正常乳腺样本相比，ITPR1在乳腺癌患者中显著降低。ITPR1 mRNA表达升高与乳腺癌患者更长的OS、DMFS、DSS和RFS密切相关。并且，接受化疗的患者中ITPR1表达水平高于未接受化疗的患者。此外，ITPR1的表达与不同类型乳腺癌中免疫细胞相关基因标志物呈正相关，尤其是在BRCA基底组织乳腺癌中。
展开英文摘要原文
ITPR1 is a key gene for autophagy, but its biological function is still unclear, and there are few studies on the correlation between ITPR1 gene expression and the occurrence and development of breast cancer.
Analyze the expression of ITPR1 through online databases such as Oncomine and TIMER. Kaplan-Meier plotter and other databases were used to evaluate the impact of ITPR1 on clinical prognosis. The expression of ITPR1 in analysis of 145 cases of breast cancer and 30 cases of adjacent normal tissue was detected by Immunohistochemistry. Statistical analysis was used to evaluate the clinical relevance and prognostic significance of abnormally expressed proteins. And the Western Blot was used to detect the expression of ITPR1 between breast cancer tissues and cells. The TIMER database studied the relationship between ITPR1 and cancer immune infiltration. And used the ROC plotter database to predict the response of ITPR1 to chemotherapy, endocrine therapy and anti-HER2 therapy in patients with breast cancer.
Compared with normal breast samples, ITPR1 was significantly lower in patients with breast cancer. And the increased expression of ITPR1 mRNA was closely related to longer overall survival (OS), distant metastasis free survival (DMFS), disease specific survival (DSS) and relapse free survival (RFS) in breast cancer. And the expression level of ITPR1 was higher in patients treated with chemotherapy than untreated patients. In addition, the expression of ITPR1 was positively correlated with related gene markers of immune cells in different types of breast cancer, especially with BRCA basal tissue breast cancer.
ITPR1 was lower expressed in breast cancer. The higher expression of ITPR1 suggested favorable prognosis for patients. ITPR1 was related to the level of immune infiltration, especially in BRCA-Basal patients. All research results indicated that ITPR1 might affect breast cancer prognosis and participate in immune regulation. In short, ITPR1 might be a potential target for breast cancer therapy.
论文信息
- 作者
- Han B、Zhen F、Zheng XS、Hu J、Chen XS
- 第一作者单位
- Department of Breast Medical Oncology, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, 150040, China.China
- 通讯作者单位
- Department of Breast Medical Oncology, Harbin Medical University Cancer Hospital, 150 Haping Road, Harbin, 150040, China. cxs1978@ems.hrbmu.edu.cn.China
- 期刊
- BMC cancer2022 Mar 21
- 原文标识
- PubMed 35313846 · DOI 10.1186/s12885-022-09410-w
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