CAR-T（CAR-T）细胞疗法在非肿瘤性疾病中的应用
Chimeric antigen receptor T (CAR-T) cell therapy in non-oncological diseases.
CAR-T（CAR-T）细胞在血液系统恶性肿瘤中的应用推动了这种免疫治疗形式的显著进展。
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
CAR-T 细胞的表型分析
英文原题：Phenotyping of CAR T cells.
查看英文原题
Phenotyping of CAR T cells.
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中文摘要
适应性免疫治疗作为癌症和其他疾病治疗的一种治疗选择不断发展；嵌合抗原受体（CAR）T细胞是适应性免疫治疗的一种类型，通过在T细胞中表达重组受体构建体来靶向表达特定抗原的细胞，是血液系统恶性肿瘤和实体瘤的一种潜在新治疗方法。输注的CAR-T 细胞的表型、活化和功能特征与CAR-T 细胞治疗的疗效相关。因此，在生物工艺过程中对CAR-T 细胞进行免疫表型表征对于控制细胞质量并最终提高抗肿瘤效果至关重要。因此，我们在本章中提出基于流式细胞术的CAR-T 细胞免疫表型分析，以确定CAR-T 亚群的表型。
展开英文摘要原文
Adaptive immunotherapy has been growing as a therapeutic alternative for cancer and other diseases treatment; chimeric antigen receptor (CAR) T cells comprise a type of adaptive immunotherapy, with recombinant receptor constructs expressed in T cells to target cells expressing specific antigens, is a potential new treatment for blood cancers and solid tumors.
The phenotypic, activation, and functional profile of infused CAR-T cells have been linked to the efficacy of CAR-T cell treatment. As a result, immunophenotypic characterization of CAR-T cells during the bioprocess is critical for controlling cell quality and, eventually, improving antitumor effectiveness.
Therefore, we propose a flow cytometry based immunophenotyping analysis of CAR-T cells in this chapter to define the phenotype of CAR-T subsets.
论文信息
- 作者
- Rathod S
- 单位
- Department of Immunology, University of Pittsburgh, Pittsburgh, PA, United States. Electronic address: sbr21@pitt.edu.United States
- 期刊
- Methods in cell biology2022
- 原文标识
- PubMed 35152999 · DOI 10.1016/bs.mcb.2021.10.001