CAR-T（CAR-T）细胞疗法在非肿瘤性疾病中的应用
Chimeric antigen receptor T (CAR-T) cell therapy in non-oncological diseases.
CAR-T（CAR-T）细胞在血液系统恶性肿瘤中的应用推动了这种免疫治疗形式的显著进展。
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
使用慢病毒载体生成 CAR-T 细胞
英文原题：Generation of CAR-T cells using lentiviral vectors.
查看英文原题
Generation of CAR-T cells using lentiviral vectors.
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中文摘要
目前，癌症免疫治疗在很大程度上聚焦于治疗性抗体和嵌合抗原受体（CAR）的开发。最近，已有两种靶向 CD19 的 CAR 获批用于治疗某些血液系统恶性肿瘤。这表明工程化 CAR-T 细胞能够产生有效的肿瘤反应。此外，数百项正在进行的临床试验正在探索基于 CAR 的方法在实体瘤中靶向肿瘤相关抗原的可行性。然而，在 CAR 修饰 T 细胞的设计和生产方面仍存在重大挑战和局限性需要解决，例如更有效的转导方法、表达和耗竭问题、可靠的体外和体内表征方法等。在此，我们描述当前使用慢病毒载体生成 CAR-T 细胞的技术，以及对其功能表征的详细方案。
展开英文摘要原文
Cancer immunotherapy is nowadays largely focused on the development of therapeutic antibodies and chimeric antigen receptors (CARs). Two CARs targeting CD19 have been approved recently for the treatment of some hematological malignancies. This demonstrates the capability of engineered CAR T cells in generating effective tumor responses.
Furthermore, several hundred ongoing clinical trials are exploring the feasibility of CAR-based approaches to target tumor-associated antigens in solid tumors.
However, there still remain significant challenges and limitations in the design and production of CAR-modified T cells that need to be addressed, such as more effective transduction methods, expression and exhaustion issues, reliable in vitro and in vivo characterization methods, etc.
Here we describe current techniques for generating CAR T cells using lentiviral vectors as well as detailed protocols for their functional characterization.
论文信息
- 作者
- Poorebrahim M、Quiros-Fernandez I、Fakhr E、Cid-Arregui A
- 第一作者单位
- Targeted Tumor Vaccines Group, Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany.Germany
- 通讯作者单位
- Targeted Tumor Vaccines Group, Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany. Electronic address: a.cid@dkfz-heidelberg.de.Germany
- 文献类型
- 非美国政府资助研究
- 期刊
- Methods in cell biology2022
- 原文标识
- PubMed 35152998 · DOI 10.1016/bs.mcb.2021.07.001