CAR-T（CAR-T）细胞疗法在非肿瘤性疾病中的应用
Chimeric antigen receptor T (CAR-T) cell therapy in non-oncological diseases.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
斑马鱼异种移植模型中 CAR-T 细胞的临床前测试
英文原题：Preclinical testing of CAR T cells in zebrafish xenografts.
查看英文原题
Preclinical testing of CAR T cells in zebrafish xenografts.
分数与星级只用于站内排序 —— 不代表疗效、安全性或个人适用性。
中文摘要
工程化CAR-T 细胞（CAR-T 细胞）已成为一种有前景的癌症免疫疗法，并已证明可有效治疗B细胞恶性肿瘤。目前，研究者正大力拓展CAR-T 细胞在其他癌症中的应用、提高CAR-T 介导的癌细胞杀伤效果，并减少可能的治疗副作用。因此，需要临床前模型评估不断涌现的新型CAR设计。传统上，人类CAR-T 细胞体内疗效通过小鼠异种移植模型研究。本文介绍一种补充性异种移植方案，在斑马鱼胚胎中测试CAR-T 细胞对抗人白血病细胞的作用。斑马鱼胚胎异种移植有望成为快速、低成本的模型，并特别适于活体成像，观察CAR-T 细胞在体内的分布和癌细胞杀伤情况。
展开英文摘要原文
Engineered chimeric antigen receptor T cells (CAR T cells) have emerged as a promising immunotherapy for cancer and have proven to be effective for B cell malignancies. Currently, great efforts are undertaken to expand the application of CAR T cells to other cancer entities, to increase the efficacy of CAR T cell-mediated killing of cancer cells and to reduce possible side effects of CAR T cell therapy.
This creates a need for preclinical models to test the many emerging novel CAR designs. Traditionally, mouse xenograft models are applied to investigate the efficacy of CAR T cells in vivo.
Here, we describe a complementing xenograft protocol for testing CAR T cells against human leukemia cells in zebrafish embryos. The embryonic zebrafish xenograft promises to be a fast and cost-efficient model and particularly offers live imaging opportunities of CAR T cell distribution and killing of cancer cells in vivo.
论文信息
- 作者
- Grissenberger S、Salzer B、Pascoal S、Wenninger-Weinzierl A、Lehner M、Distel M
- 第一作者单位
- St. Anna Children's Cancer Research Institute, Innovative Cancer Models, Vienna, Austria.Austria
- 通讯作者单位
- St. Anna Children's Cancer Research Institute, Innovative Cancer Models, Vienna, Austria. Electronic address: martin.distel@ccri.at.Austria
- 文献类型
- 非美国政府资助研究
- 期刊
- Methods in cell biology2022
- 原文标识
- PubMed 35152991 · DOI 10.1016/bs.mcb.2021.07.002