RNF43 p.G659fs 通过 PI3K/AKT/mTOR 信号通路和 HLA-E 上调导致 MSI-high 结直肠癌中 NK 细胞功能障碍
RNF43 p.G659fs leads to natural killer cell dysfunction in MSI-high colorectal cancer through PI3K/AKT/mTOR signaling and HLA-E up-regulation.
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
台湾灵芝胞外多糖通过免疫调节抑制肿瘤生长
英文原题：Ganoderma formosanum Exopolysaccharides Inhibit Tumor Growth via Immunomodulation.
查看英文原题
Ganoderma formosanum Exopolysaccharides Inhibit Tumor Growth via Immunomodulation.
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中文摘要
台湾特有药用真菌台湾灵芝（Ganoderma formosanum, GF）经液体发酵可产生富含β-葡聚糖的胞外多糖（GF-EPS），本研究探讨了GF-EPS在Lewis肺癌细胞（LLC1）荷瘤小鼠模型中的抗肿瘤作用。在预防模型中，小鼠在接种LLC1前经口给予GF-EPS；在治疗模型中，小鼠在肿瘤细胞接种后五天开始经口给予GF-EPS。记录小鼠肿瘤大小和体重。处死后，使用流式细胞术分析淋巴细胞亚群。使用脾组织分析细胞因子mRNA表达。结果显示，GF-EPS（80 mg/kg）在预防模型和治疗模型中均有效抑制LLC1肿瘤生长。给予GF-EPS增加了脾脏中NK 细胞的比例，并激活了多种细胞因子的基因表达。我们的结果提供证据表明，GF-EPS通过免疫调节促进荷瘤小鼠的肿瘤抑制。
展开英文摘要原文
Ganoderma formosanum (GF) is a medicinal mushroom endemic to Taiwan. Previous research established the optimal culture conditions to produce exopolysaccharide rich in β-glucan (GF-EPS) from submerged fermentation of GF. The present study investigated the antitumor effects of GF-EPS in a Lewis lung carcinoma cell (LLC1) tumor-bearing mice model. In the preventive model, GF-EPS was orally administered to mice before LLC1 injection. In the therapeutic model, GF-EPS oral administration was initiated five days after tumor cell injection.
The tumor size and body weight of the mice were recorded. After sacrifice, the lymphocyte subpopulation was analyzed using flow cytometry. Spleen tissues were used to analyze cytokine mRNA expression. The results showed that GF-EPS (80 mg/kg) effectively suppressed LLC1 tumor growth in both the preventive and therapeutic models. GF-EPS administration increased the proportion of natural killer cells in the spleen and activated gene expression of several cytokines.
Our results provide evidence that GF-EPS promotes tumor inhibition through immunomodulation in tumor-bearing mice.
论文信息
- 作者
- Kuo HC、Liu YW、Lum CC、Hsu KD、Lin SP、Hsieh CW、Lin HW、Lu TY
- 第一作者单位
- Department of Nursing, Division of Basic Medical Sciences, Chang Gung University of Science and Technology, Chiayi 613, Taiwan.Taiwan
- 通讯作者单位
- Institute of Biotechnology, National Taiwan University, Taipei 106, Taiwan.Taiwan
- 期刊
- International journal of molecular sciences2021 Oct 19
- 原文标识
- PubMed 34681911 · DOI 10.3390/ijms222011251
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