CAR-T（CAR-T）细胞疗法在非肿瘤性疾病中的应用
Chimeric antigen receptor T (CAR-T) cell therapy in non-oncological diseases.
CAR-T（CAR-T）细胞在血液系统恶性肿瘤中的应用推动了这种免疫治疗形式的显著进展。
CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
通过聚焦超声控制肿瘤内 CAR-T 细胞的活性
英文原题：Control of the activity of CAR-T cells within tumours via focused ultrasound.
查看英文原题
Control of the activity of CAR-T cells within tumours via focused ultrasound.
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中文摘要
聚焦超声能以安全、非侵入性的方式将能量传递至数厘米深的组织内。在此，我们表明，肿瘤内CAR-T 细胞（CAR-T 细胞）的遗传学和细胞功能可通过短脉冲聚焦超声产生的热量，经由受热休克蛋白启动子控制的CAR盒实现可逆调控。在皮下肿瘤小鼠中，与不可诱导的CAR-T 细胞相比，局部注射带有可诱导CAR的T细胞并通过磁共振成像引导的聚焦超声激活，减轻了靶向非肿瘤活性并增强了对肿瘤生长的抑制。对工程化T细胞激活的声遗传学控制可能有助于设计更安全的细胞疗法。
展开英文摘要原文
Focused ultrasound can deliver energy safely and non-invasively into tissues at depths of centimetres.
Here we show that the genetics and cellular functions of chimeric antigen receptor T cells (CAR-T cells) within tumours can be reversibly controlled by the heat generated by short pulses of focused ultrasound via a CAR cassette under the control of a promoter for the heat-shock protein.
In mice with subcutaneous tumours, locally injected T cells with the inducible CAR and activated via focused ultrasound guided by magnetic resonance imaging mitigated on-target off-tumour activity and enhanced the suppression of tumour growth, compared with the performance of non-inducible CAR-T cells. Acoustogenetic control of the activation of engineered T cells may facilitate the design of safer cell therapies.
论文信息
- 作者
- Wu Y、Liu Y、Huang Z、Wang X、Jin Z、Li J、Limsakul P、Zhu L
- 第一作者单位
- Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.United States
- 通讯作者单位
- Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA. yiw015@eng.ucsd.edu.United States
- 文献类型
- 美国 NIH 资助研究
- 期刊
- Nature biomedical engineering2021 Nov
- 原文标识
- PubMed 34385696 · DOI 10.1038/s41551-021-00779-w
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