CELL INTELLIGENCE · 肿瘤细胞治疗研究

We developed B7-H3-targeting TRuC-T cells and incorporated NF- B- or NFAT-responsive transcriptional elements into the TRuC construct. Using flow cytometry, luciferase assays, and in vitro / vivo functional studies, we evaluated whether TRuC-T cells exert cytotoxicity via NF- B and NFAT signaling pathways.

B7-H3 TRuC-T cells effectively suppressed GBM growth both in vitro and in vivo . Co-culture of TRuC-T cells carrying NF- B- or NFAT-reporters with target cells induced GFP expression, indicating pathway activation. DISCUSSION: Upon engagement with B7-H3, both NFAT and NF- B signaling pathways are activated and participate in the regulation of B7-H3-targeting TRuC-T cells. Our data demonstrate that NF- B/NFAT transcriptional reporters integrated into these TRuC constructs can be utilized to monitor target recognition and may serve as early indicators of cytotoxic efficacy. Overall, this system suggests a potential avenue for GBM therapy and offers an initial framework for the functional assessment of TRuC-T cell therapeutic potency.