肿瘤细胞治疗研究
英文原题：Advances in ex vivo mRNA-based gene transfer for cell-based cancer immunotherapy.
信使RNA（mRNA）电穿孔是一种离体非整合型基因转移技术，用于基于免疫细胞的癌症试验中，以瞬时向免疫细胞提供多种蛋白质。
信使RNA电转染是一种离体非整合型基因转移技术，用于基于免疫细胞的癌症试验中，以瞬时向免疫细胞提供多种蛋白质。该技术已被用于改造树突状细胞和B细胞使其表达肿瘤相关抗原，以增强癌症患者的免疫系统，并利用免疫受体重定向T细胞和NK 细胞的抗肿瘤活性。尽管通过mRNA电转染进行基因递送仅导致目标蛋白的瞬时表达，但与使用病毒载体的稳定表达方法相比，许多研究者和临床医生认为它是一种可行、灵活且安全的技术。在这篇综述中，我们讨论了mRNA电转染用于基因转移的功效，并评估了该技术在癌症免疫治疗中重定向和增强针对各种肿瘤抗原的免疫应答方面的优势和局限性。
Electroporation of messenger RNA (mRNA) is an ex vivo non-integrating gene transfer technique used in immune-cell-based trials for cancer to transiently supply immune cells with multiple proteins. This technique has been used to engineer dendritic cells and B cells with tumor-associated antigens to boost the immune system of cancer patients and to redirect the anti-tumor activity of T cells and natural killer cells with immune receptors. Although gene delivery via mRNA electroporation results only in transient expression of the protein of interest, many investigators and clinicians consider it as a feasible, flexible, and safe technique, compared with stable expression methods using viral vectors. In this review, we discuss the efficacy of mRNA electroporation for gene transfer and assess the strengths and limitations of this technique for redirecting and boosting immune responses against various tumor antigens in cancer immunotherapy.
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