间皮素作为癌症免疫治疗的生物标志物和治疗靶点
Mesothelin as Biomarker and Therapeutic Target for Immunotherapy in Cancer.
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CELL INTELLIGENCE · 肿瘤细胞治疗研究
肿瘤细胞治疗研究
胃癌活检组织样本中 TIL（肿瘤浸润淋巴细胞）的培养与扩增可行
英文原题：Culture and Expansion of Tumor-Infiltrating Lymphocyte From Biopsy Tissue Samples of Gastric Cancer Are Feasible.
查看英文原题
Culture and Expansion of Tumor-Infiltrating Lymphocyte From Biopsy Tissue Samples of Gastric Cancer Are Feasible.
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中文摘要
对实体瘤实施TIL（肿瘤浸润淋巴细胞）过继转移已取得有希望的临床结果。TIL 临床试验通常使用手术切除的组织分离 TIL；但某些组织难以手术切除，如果微创方法能够获得相当数量的 TIL，可能更具优势。本研究测试从转移性胃癌患者的内镜活检肿瘤中培养和扩增 TIL 的微创方法。所有肠型胃癌活检样本均成功培养出 TIL，而弥漫型胃癌的 TIL 群体生长较慢。肠型胃癌活检样本（n=9）的初始 TIL 生长速度快于弥漫型胃癌活检样本（n=4；P=0.021）。接受检测的 4 份活检来源 TIL 均通过快速扩增方案扩增至超过 10¹⁰ 个。活检来源 TIL 的扩增情况和流式细胞术（FACS）表型与胃切除术标本培养的 TIL 相当。因此，可从转移性胃癌患者的内镜活检肿瘤中成功培养并扩增达到临床级标准的 TIL。
展开英文摘要原文
Adoptive transfer of tumor-infiltrating lymphocytes (TILs) achieves promising clinical results in solid tumors. TIL trials use surgically resected tissue to isolate TILs.
However, certain tissues are difficult to surgically resect, and a minimally invasive approach might be advantageous if it could achieve similar TIL yields.
We tested a minimally invasive approach to culture and expand TILs using endoscopic biopsy tumors from patients with metastatic gastric cancer (GC). TILs were successfully cultured from all of the intestinal GC biopsies, while TIL populations had slow growth from diffuse GCs.
Initial TIL growth was faster in intestinal GC biopsies (n=9) than in diffuse GC biopsies (n=4) ( P =0. 021). All 4 biopsy TILs tested were expanded >10 10 by our rapid expansion protocol. Biopsy TILs demonstrated equivalent expansion and FACS profiles to TILs cultured from gastrectomy.
Thus, clinical-grade TILs can be successfully cultured and expanded from endoscopic biopsy tumors in patients with metastatic GC.
论文信息
- 作者
- Park B、Park H、Seo MJ、Kim SW、Baek IP、Kim YW、Kim HK
- 单位
- National Cancer Center, Goyang-si, Gyeonggi-do, Republic of Korea.South Korea
- 期刊
- Journal of immunotherapy (Hagerstown, Md. : 1997)2026 Jul-Aug 01
- 原文标识
- PubMed 42102352 · DOI 10.1097/CJI.0000000000000607